使用小亚基核糖体DNA（Ssu-rDNA）扩增的聚合酶链反应-限制性片段长度多态性分析（PCR-RFLP）：在牛不同锥虫种类混合感染诊断中的适用性

PCR-RFLP using Ssu-rDNA amplification: applicability for the diagnosis of mixed infections with different trypanosome species in cattle.

作者信息

Delespaux V, Ayral F, Geysen D, Geerts S

机构信息

Institute of Tropical Medicine, Nationalestraat 155, B-2000 Antwerp, Belgium.

出版信息

Vet Parasitol. 2003 Nov 14;117(3):185-93. doi: 10.1016/j.vetpar.2003.08.004.

DOI:10.1016/j.vetpar.2003.08.004

PMID:14630427

Abstract

The use of a single restriction fragment length polymorphism (RFLP)-PCR assay which is able to characterise all important bovine trypanosome species was evaluated for the detection of mixed infections with Trypanosoma brucei brucei, Trypanosoma theileri, Trypanosoma congolense and Trypanosoma vivax. Results showed that mixed infections are detectable at a minimum ratio of 2%/98% of standardised DNA solutions with a concentration of 10 ng ml(-1). All mixed infections gave clear profiles that could be easily differentiated except with T. theileri and T. congolense where the T. theileri band was concealed by the T. congolense profile.

摘要

评估了一种能够鉴定所有重要牛锥虫物种的单一限制性片段长度多态性（RFLP）-PCR检测方法，用于检测布氏布氏锥虫、泰勒锥虫、刚果锥虫和活泼锥虫的混合感染。结果表明，对于浓度为10 ng ml⁻¹的标准化DNA溶液，以2%/98%的最低比例可检测到混合感染。除泰勒锥虫和刚果锥虫外，所有混合感染均给出清晰的图谱，易于区分，在泰勒锥虫和刚果锥虫的混合感染中，泰勒锥虫条带被刚果锥虫图谱掩盖。