Segal R A, Takahashi H, McKay R D
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Neuron. 1992 Dec;9(6):1041-52. doi: 10.1016/0896-6273(92)90064-k.
Neurotrophins and their receptors are widespread in the developing and mature CNS. Identifying the differentiation state of neurotrophin-responsive cells provides a basis for understanding the developmental functions of these factors. Studies using dissociated and organotypic cultures of rat cerebellum demonstrated that the neurotrophins brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) affect developing granule cells at distinct stages in differentiation. While early granule neurons in the external germinal layer responded to BDNF, more mature granule cells responded to NT-3. BDNF, but not NT-3, enhanced survival of granule cells in cultures of embryonic cerebella. Thus, BDNF and NT-3 have distinct sequential functions that are likely to be critical in the development of the cerebellum. BDNF may promote the initial commitment, while NT-3 may direct the subsequent maturation of granule cells.
神经营养因子及其受体广泛分布于发育中的和成熟的中枢神经系统。确定神经营养因子反应性细胞的分化状态为理解这些因子的发育功能提供了基础。使用大鼠小脑的解离培养物和器官型培养物进行的研究表明，神经营养因子脑源性神经营养因子（BDNF）和神经营养因子-3（NT-3）在分化的不同阶段影响发育中的颗粒细胞。虽然外颗粒层中的早期颗粒神经元对BDNF有反应，但更成熟的颗粒细胞对NT-3有反应。BDNF而非NT-3可提高胚胎小脑培养物中颗粒细胞的存活率。因此，BDNF和NT-3具有不同的顺序性功能，这可能对小脑的发育至关重要。BDNF可能促进初始定向分化，而NT-3可能指导颗粒细胞的后续成熟。
