Zhan Yu-tao, Wei Lai, Chen Hong-song, Cong Xu, Fei Ran, Wang Yu
Hepatology Institute, People's Hospital, Peking University, Beijing 100044, China.
Zhonghua Gan Zang Bing Za Zhi. 2003 Nov;11(11):673-5.
To observe the differentiation of bone marrow stem cells in rat hepatic fibrogenesis environment into hepatocytes.
Rat hepatic fibrosis was induced by subcutaneous injection of CCl4. Bone marrow stem cells with Thy positive, CD3 and CD45RA negative were enriched from the bone marrow by fluorescence-activated cell sorting. The bone marrow stem cells were labeled with PKH26-GL, and then autotransplanted. After six weeks, albumin, ck8 and a-smooth muscle actin expression were determined by immunocytochemistry.
The PKH26-GL labeled cells expressed albumin and ck8, but did not express a-smooth muscle actin in hepatic fibrogenesis environment.
Bone marrow stem cells in hepatic fibrogenesis environment can differentiate into hepatocytes, but can't differentiate into myofibroblasts
观察大鼠肝纤维化形成环境中骨髓干细胞向肝细胞的分化情况。
通过皮下注射四氯化碳诱导大鼠肝纤维化。采用荧光激活细胞分选技术从骨髓中富集 Thy 阳性、CD3 和 CD45RA 阴性的骨髓干细胞。用 PKH26-GL 标记骨髓干细胞，然后进行自体移植。六周后，通过免疫细胞化学检测白蛋白、ck8 和α-平滑肌肌动蛋白的表达。
在肝纤维化形成环境中，PKH26-GL 标记的细胞表达白蛋白和 ck8，但不表达α-平滑肌肌动蛋白。
肝纤维化形成环境中的骨髓干细胞可分化为肝细胞，但不能分化为肌成纤维细胞。
