Yang Shu-Fang, Tay Joo-Hwa, Liu Yu
Environmental Engineering Research Centre, School of Civil and Environmental Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore.
J Biotechnol. 2003 Dec 5;106(1):77-86. doi: 10.1016/j.jbiotec.2003.07.007.
Microbial granules were developed at different substrate N/COD ratios in sequencing batch reactors (SBR). Results showed that heterotrophic, nitrifying, and denitrifying populations could peacefully co-exist in microbial granules, while increased substrate N/COD ratio led to significant shifts among three populations in granules. Enhanced activities of nitrifying and denitrifying populations were obtained in microbial granules developed at high substrate N/COD ratios, however, heterotrophic populations in granules tended to decrease with the increase of substrate N/COD ratio. It was found that dissolved oxygen (DO) concentration had a pronounced effect on the efficiency of denitrification by microbial granules, meanwhile the results also indicated that a certain mixing power would be provided to ensure mass transfer between liquid and granules during denitrification. It was demonstrated that complete organics and nitrogen removal can be achieved in single granule-based SBR with high efficiency and stable performance. This is the first study to show the capability of microbial granules in simultaneous removal of organic carbon and nitrogen from wastewater.
在序批式反应器（SBR）中，以不同的底物氮/化学需氧量（N/COD）比值培养微生物颗粒。结果表明，异养菌、硝化菌和反硝化菌种群能够在微生物颗粒中和平共存，而底物N/COD比值的增加导致颗粒中这三种菌群发生显著变化。在高底物N/COD比值下培养的微生物颗粒中，硝化菌和反硝化菌种群的活性增强，然而，随着底物N/COD比值的增加，颗粒中的异养菌种群趋于减少。研究发现，溶解氧（DO）浓度对微生物颗粒的反硝化效率有显著影响，同时结果还表明，在反硝化过程中需要提供一定的搅拌功率以确保液体与颗粒之间的传质。结果表明，基于单个颗粒的SBR能够高效、稳定地实现有机物和氮的完全去除。这是第一项展示微生物颗粒同时去除废水中有机碳和氮能力的研究。
