Půza V V, Novák L
Zahn Mund Kieferheilkd Zentralbl. 1977;65(8):863-6.
This paper discribes a method that enables a qualitative analysis of the biological effects of stomatological materials upon living cells cultivated in vitro to be made. This method allows to make photographic records, at regular intervals of time, of those cells which are originally in a state of suspension, then start to adhere to the bottom of the vessel used for purposes of cultivation, when they become flat and wider. This method takes of the capability of cytoplasmic membranes to respond to any kind of chemical or physical noxa. Quick-motion pictures taken at regular intervals of time using ciné film provide for a quantitative differentiation and determination of the ratio of dilated to nondilated cells, which can be analyzed statistically.
本文描述了一种方法，该方法能够对口腔材料对体外培养的活细胞的生物学效应进行定性分析。这种方法可以每隔一定时间对那些最初处于悬浮状态的细胞进行摄影记录，这些细胞随后开始附着在用于培养目的的容器底部，当它们变得扁平且更宽时。该方法利用了细胞质膜对任何化学或物理损伤作出反应的能力。使用电影胶片每隔一定时间拍摄的快动作影片能够对扩张细胞与未扩张细胞的比例进行定量区分和测定，并可进行统计分析。
