Brito M J, Filipe M I, Morris R W
Department of Histopathology, Hosp Sta Marta, Lisbon, Portugal.
Eur J Cancer Prev. 1992 Oct;1(6):429-35. doi: 10.1097/00008469-199210000-00006.
Previous reports using [3H]thymidine have shown altered patterns of cell proliferation in gastric carcinogenesis. In this study we applied in vitro bromodeoxyuridine (BrdU) uptake and immunohistochemistry to tissue from carcinoma, its adjacent mucosa and distant mucosa (n = 16) cases and 10 normal control cases. The labelling index (LI) was calculated for the upper, neck/isthmus and lower gland compartments and in 10 random fields/carcinoma. The LI was greater in the carcinoma than in either the adjacent or far mucosa or the controls (p < 0.001). No difference was observed between the non-carcinoma areas. However, the LI varied significantly between the gland compartments within each area (p < 0.001). In the normal control cases, the location of the proliferating cells was limited to the neck/isthmus region and perhaps the most significant observation was the change of location and expansion of the proliferative zone towards the surface in the normal mucosa adjacent to carcinoma compared with distant mucosa and controls (p < 0.001). These altered patterns of cell proliferation may be an important aspect of the precancerous potential of field changes in gastric carcinoma patients and could help patient follow-up and serve as a marker in monitoring intervention studies.
以往使用[3H]胸腺嘧啶核苷的报告显示，胃癌发生过程中细胞增殖模式发生了改变。在本研究中，我们对16例癌组织、其邻近黏膜和远处黏膜以及10例正常对照病例的组织进行了体外溴脱氧尿苷（BrdU）摄取和免疫组织化学检测。计算了上腺区、颈/峡部和下腺区以及10个随机视野/癌组织中的标记指数（LI）。癌组织中的LI高于邻近或远处黏膜以及对照组（p < 0.001）。非癌区域之间未观察到差异。然而，每个区域内腺区之间的LI差异显著（p < 0.001）。在正常对照病例中，增殖细胞的位置局限于颈/峡部区域，也许最显著的观察结果是，与远处黏膜和对照组相比，癌旁正常黏膜中增殖区的位置发生改变并向表面扩展（p < 0.001）。这些细胞增殖模式的改变可能是胃癌患者场效应癌前潜能的一个重要方面，有助于患者随访，并可作为监测干预研究的一个标志物。
