Lynch D A, Clarke A M, Jackson P, Axon A T, Dixon M F, Quirke P
Centre for Digestive Diseases, General Infirmary at Leeds.
J Clin Pathol. 1994 Feb;47(2):122-5. doi: 10.1136/jcp.47.2.122.
To compare proliferating cell nuclear antigen (PCNA) and MIB-1 with bromodeoxyuridine (BrdU) pulse labelling, a specific marker of cell proliferation, in endoscopic gastric biopsy specimens.
Twenty four biopsy specimens were obtained from 12 patients: 10 antral and eight body specimens were suitable. Each specimen was routinely processed and stained with haematoxylin and eosin. A modified Giemsa stain was used to detect the presence of Helicobacter pylori. Sections of the specimens were labelled with BrdU, MIB-1, and PC10. Gastric mucosa specimens were divided into three zones. The numbers of positively staining nuclei for 500 epithelial cell nuclei were counted in each zone and expressed as a percentage.
The proportion of PCNA positive cells (range 0-90%) was much greater in all specimens (10 antrum, eight body). BrdU positive cells were virtually all confined to zone 2 (0-17% cells in this zone were positive) (zone 1 = surface and gastric pit, zone 2 = isthmus, zone 3 = gland base), while PCNA positive cells were present in all three zones (1 = 23-90%, 2 = 43-90%, 3 = 0-74%). Spearman's rank coefficient correlation of 0.57 confirmed that the percentage of positively staining cells varied in the same direction for both PCNA and BrdU (p < 0.001). PCNA, however, was overexpressed in all zones of the gastric epithelium compared with BrdU. In 38 biopsy specimens from 19 patients, of which 14 antrum and 11 body were suitable, the proportion of MIB-1 positive cells (0-59%) was greater than BrdU in most. As with BrdU labelling, the MIB-1 positive cells were confined to zone 2 (zone 1 = 1-11%); zone 2 = 21-59%; zone 3 = 0-13%) and the coefficient correlation for MIB-1 and BrdU was 0.63 (p < 0.001).
MIB-1 accurately reflects the S-phase fraction in gastric mucosa, determined by BrdU labelling in conventionally processed gastric biopsy material. Caution is needed in the interpretation of PCNA labelling detected by PC10, which should not be accepted uncritically as a marker of cell proliferation in paraffin wax embedded material.
在内镜下胃活检标本中，将增殖细胞核抗原（PCNA）和MIB-1与细胞增殖的特异性标志物溴脱氧尿苷（BrdU）脉冲标记法进行比较。
从12例患者获取24份活检标本：10份胃窦标本和8份胃体标本符合要求。每份标本常规处理并用苏木精和伊红染色。采用改良吉姆萨染色检测幽门螺杆菌的存在。标本切片用BrdU、MIB-1和PC10进行标记。胃黏膜标本分为三个区域。对每个区域500个上皮细胞核中阳性染色核的数量进行计数并表示为百分比。
所有标本（10份胃窦，8份胃体）中PCNA阳性细胞的比例（范围0 - 90%）要高得多。BrdU阳性细胞几乎都局限于区域2（该区域0 - 17%的细胞为阳性）（区域1 = 表面和胃小凹，区域2 = 峡部，区域3 = 腺底部），而PCNA阳性细胞存在于所有三个区域（区域1 = 23 - 90%，区域2 = 43 - 90%，区域3 = 0 - 74%）。Spearman等级系数相关性为0.57，证实PCNA和BrdU阳性染色细胞的百分比在相同方向变化（p < 0.001）。然而，与BrdU相比，PCNA在胃上皮的所有区域均过度表达。在来自19例患者的38份活检标本中，其中14份胃窦和11份胃体符合要求，大多数情况下MIB-1阳性细胞的比例（0 - 59%）高于BrdU。与BrdU标记一样，MIB-1阳性细胞局限于区域2（区域1 = 1 - 11%；区域2 = 21 - 59%；区域3 = 0 - 13%），MIB-1和BrdU的系数相关性为0.63（p < 0.001）。
MIB-1准确反映了通过常规处理的胃活检材料中BrdU标记所确定的胃黏膜S期分数。在解释通过PC10检测到的PCNA标记时需要谨慎，在石蜡包埋材料中不应不加批判地将其作为细胞增殖的标志物。
