Davidek Tomas, Clety Nathalie, Devaud Stéphanie, Robert Fabien, Blank Imre
Centres R&D Nestlé SAS, Amiens, P.O. Box 47, 80800 Aubigny, France.
J Agric Food Chem. 2003 Dec 3;51(25):7259-65. doi: 10.1021/jf034794n.
A new analytical setup allowing the simultaneous analysis of precursors and products of the Maillard reaction is described. It is based on high-performance anion exchange chromatography with electrochemical (ECD) and diode array detectors (DAD) coupled in series. Chromatography and detection were optimized to permit simultaneous monitoring of compounds relevant to the Maillard reaction, such as the sugar, the amino acid, and the corresponding Amadori compound as well as the cyclic intermediates 5-(hydroxymethyl)-2-furaldehyde, maltol, and 2,3-dihydro-3,5-dihydroxy-6-methyl-4(H)-pyran-4-one. Separation was achieved on a CarboPac PA-1 column using a gradient of sodium acetate in aqueous sodium hydroxide. The Amadori compound, glucose, and glycine were monitored by an ECD operating in the integrated amperometry mode. The number of analyzed compounds was further increased by coupling the ECD with a DAD for the analysis of ultraviolet-active constituents. This method was successfully applied to model Maillard reaction mixtures based on glucose and glycine.
本文描述了一种新的分析装置，可同时分析美拉德反应的前体和产物。该装置基于串联电化学（ECD）检测器和二极管阵列检测器（DAD）的高效阴离子交换色谱法。对色谱和检测进行了优化，以同时监测与美拉德反应相关的化合物，如糖类、氨基酸、相应的阿马多里化合物以及环状中间体5-（羟甲基）-2-糠醛、麦芽酚和2,3-二氢-3,5-二羟基-6-甲基-4（H）-吡喃-4-酮。在CarboPac PA-1柱上，使用氢氧化钠水溶液中的醋酸钠梯度实现分离。采用积分安培法操作的ECD监测阿马多里化合物、葡萄糖和甘氨酸。通过将ECD与DAD联用分析紫外活性成分，进一步增加了可分析化合物的数量。该方法成功应用于基于葡萄糖和甘氨酸的美拉德反应模型混合物。
