College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
J Dairy Sci. 2012 Nov;95(11):6379-83. doi: 10.3168/jds.2012-5623. Epub 2012 Sep 7.
A simultaneous analysis of reducing sugars and 5-hydroxymethyl-2-furaldehyde of the Maillard reaction products was detailed. It was based on a high performance anion exchange chromatography with electrochemical detector system and an HPLC with refractive index detector. Results showed that high performance anion exchange chromatography with electrochemical detector using a CarboPac PA-1 column (Dionex Corp., Sunnyvale, CA) was more suitable for reducing sugars and 5-hydroxymethyl-2-furaldehyde determination, especially for trace analysis. The lowest detectable limit of reducing sugars and 5-hydroxymethyl-2-furaldehyde was 0.00005 mol/L in this experiment. However, HPLC with a refractive index detector always produces a tailing peak for 5-hydroxymethyl-2-furaldehyde, and mannose and fructose cannot be absolutely separated. The results of the present study could provide a more sensitive means for 5-hydroxymethyl-2-furaldehyde and reducing sugar detection.
详细描述了美拉德反应产物中还原糖和 5-羟甲基-2-糠醛的同时分析。该方法基于高效阴离子交换色谱电化学检测系统和高效液相色谱折射指数检测系统。结果表明，采用 CarboPac PA-1 柱（Dionex Corp.，Sunnyvale，CA）的高效阴离子交换色谱电化学检测法更适合还原糖和 5-羟甲基-2-糠醛的测定，尤其是痕量分析。在本实验中，还原糖和 5-羟甲基-2-糠醛的最低检测限为 0.00005 mol/L。然而，高效液相色谱折射指数检测法总是会产生 5-羟甲基-2-糠醛的拖尾峰，并且甘露糖和果糖不能完全分离。本研究结果可为 5-羟甲基-2-糠醛和还原糖的检测提供更灵敏的方法。
