Sedlácková M, Soucek A, Mucksová J
Klinika dĕtského a dorostového lékarství 1. LF UK, Praha.
Cesk Pediatr. 1992 Oct;47(10):577-80.
Serum antibodies anti-Helicobacter pylori (H.p.) which are specific against antigenic determinants of the bacterial body, were determined in 183 non-ulcer dyspeptic children and adolescents, aged 7- to 20 years. 108 patients (59%) of the same group underwent fibroendoscopy for direct detection of H.p. in bioptic specimens of the stomach and duodenum microbiological methods. Discrimination analysis revealed a overall positive correlation between the results of the two methods: P < 0.01. For detection of specific IgG antibodies against H.p., evaluation by the P/N ratio was used, i.e. the absorbance of the examined serum (P) was compared with the mean absorbance of three negative serum controls (N). The specificity of the ELISA test in this examination was 96.1%, the sensitivity of 90.3%. The mentioned ELISA method for detection of specific IgG antibodies against H.p. is comparable with direct microbiological prove of H.p. presence in mucus specimens in our group examined. It is a very important non-invasive method useful for primary detection of H.p. infection.
在183名7至20岁的非溃疡性消化不良儿童和青少年中，检测了针对菌体抗原决定簇的抗幽门螺杆菌（H.p.）血清抗体。该组中的108名患者（59%）接受了纤维内镜检查，通过微生物学方法直接检测胃和十二指肠活检标本中的H.p.。判别分析显示两种方法的结果之间存在总体正相关：P < 0.01。对于检测抗H.p.的特异性IgG抗体，采用P/N比值进行评估，即将检测血清的吸光度（P）与三个阴性血清对照的平均吸光度（N）进行比较。本次检查中ELISA试验的特异性为96.1%，敏感性为90.3%。上述用于检测抗H.p.特异性IgG抗体的ELISA方法与我们所检查组中黏液标本中H.p.存在的直接微生物学证据相当。它是一种非常重要的非侵入性方法，可用于H.p.感染的初步检测。
