Kristek F, Fáberová V, Varga I
Institute of Normal and Pathological Physiology, Slovak Academy of Sciences, Sienkiewiczova 1, 813 71 Bratislava, Slovak Republic.
Physiol Res. 2003;52(6):709-17.
We studied the effects of long-term administration of molsidomine and pentaerythrityl tetranitrate (PETN) on the cardiovascular system of spontaneously hypertensive rats (SHR). One control and three experimental groups of 10-week-old animals were used: 1) control Wistar rats, 2) SHR, 3) SHR treated with molsidomine in tap water (100 mg/kg/day, by gavage), and 4) SHR treated with PETN in tap water (200 mg/kg/day, by gavage). After six weeks, the content of cGMP in platelets and NO synthase (NOS) activity in aortas were evaluated in the experimental groups. For morphological evaluation the rats were perfused at 120 mm Hg with a glutaraldehyde fixative and the arteries were processed for electron microscopy. Blood pressure and heart weight/body weight ratio (HW/BW) were increased in all experimental groups with respect to the controls. HW/BW was lower in the molsidomine group in comparison to both SHR and PETN-treated group. The platelet content of cGMP was increased and the activity of NOS in the aortas was decreased in the molsidomine and PETN-treated groups. Wall thickness and cross-sectional area of thoracic aorta, carotid artery and coronary artery were increased similarly in all experimental groups compared to the controls, but there were no differences among the experimental groups. We summarize that long-term administration of exogenous NO donors did not improve pathological changes of the cardiovascular system in SHR.
我们研究了长期给予吗多明和季戊四醇四硝酸酯（PETN）对自发性高血压大鼠（SHR）心血管系统的影响。使用了一组对照和三组10周龄的实验动物：1）对照Wistar大鼠，2）SHR，3）用自来水中的吗多明处理的SHR（100 mg/kg/天，经口灌胃），以及4）用自来水中的PETN处理的SHR（200 mg/kg/天，经口灌胃）。六周后，评估实验组血小板中cGMP的含量和主动脉中一氧化氮合酶（NOS）的活性。为了进行形态学评估，以120 mmHg的压力用戊二醛固定剂灌注大鼠，并对动脉进行电子显微镜处理。与对照组相比，所有实验组的血压和心脏重量/体重比（HW/BW）均升高。吗多明组的HW/BW低于SHR组和PETN处理组。吗多明组和PETN处理组的血小板cGMP含量增加，主动脉中NOS的活性降低。与对照组相比，所有实验组胸主动脉、颈动脉和冠状动脉的壁厚和横截面积均类似增加，但实验组之间无差异。我们总结认为，长期给予外源性一氧化氮供体并不能改善SHR心血管系统的病理变化。
