Hayashi H, Arai K, Sato O, Shimaya A, Sai Y, Ohkuma S
Department of Biochemistry, Faculty of Pharmaceutical Sciences, Kanazawa University, Ishikawa, Japan.
Chem Pharm Bull (Tokyo). 1992 Oct;40(10):2783-6. doi: 10.1248/cpb.40.2783.
At least three types of vanadate-insensitive membranous ATPase were identified on rat liver lysosomes: bafilomycin A1-sensitive Mg(2+)-ATPase (H(+)-ATPase), N-ethylmaleimide (NEM)-sensitive but bafilomycin A1-insensitive Mg(2+)-ATPase (ATPase I), and NEM-insensitive Ca2+/Mg(2+)-ATPase (ATPase II). They showed different sensitivity to chemicals and ions with apparent molecular masses of 700-800, 500-650, and 360 kDa, respectively. Of these membranous ATPases, H(+)-ATPase seemed to constitute only one tenth of the ATPase activity on rat liver lysosomes and to be the only ATPase that exposed its active site to the cytoplasmic side of the lysosomal membranes.
在大鼠肝脏溶酶体上至少鉴定出三种对钒酸盐不敏感的膜ATP酶：巴弗洛霉素A1敏感的Mg(2+)-ATP酶（H(+)-ATP酶）、N-乙基马来酰亚胺（NEM）敏感但巴弗洛霉素A1不敏感的Mg(2+)-ATP酶（ATP酶I）和NEM不敏感的Ca2+/Mg(2+)-ATP酶（ATP酶II）。它们对化学物质和离子表现出不同的敏感性，表观分子量分别为700-800、500-650和360 kDa。在这些膜ATP酶中，H(+)-ATP酶似乎仅占大鼠肝脏溶酶体上ATP酶活性的十分之一，并且是唯一将其活性位点暴露于溶酶体膜细胞质侧的ATP酶。
