Kamiya H, Sakaguchi T, Murata N, Fujimuro M, Miura H, Ishikawa H, Shimizu M, Inoue H, Nishimura S, Matsukage A
Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
Chem Pharm Bull (Tokyo). 1992 Oct;40(10):2792-5. doi: 10.1248/cpb.40.2792.
DNA templates containing a modified base (O6-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase beta and mouse DNA polymerase alpha-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.
