Tsarouhas Vasilios, Gullberg Urban, Lagercrantz Ulf
Department of Plant Biology, Swedish University of Agricultural Sciences, Uppsala, Sweden.
Hereditas. 2003;138(3):172-8. doi: 10.1034/j.1601-5223.2003.01695.x.
Dormancy release is an important phenological stage, which determines plant growth and survival in northern temperate regions. Spring bud flushing was studied in a Salix pedigree (n=82) derived from a cross between the male hybrid clone "Björn" (Salix viminalis x Salix schwerinii) and the female clone "78183" (Salix viminalis). The timing of bud flush was recorded outdoors in two consecutive years (1998, 1999) and indoor in the spring of 1998. Timing of bud flush was found to be under moderately strong genetic control (clonal mean heritabilities ranging from 0.43 to 0.72). Phenotypic correlations between height growth and bud flushing were negative but non-significant (r=0.1-0.3). Using a Salix linkage map composed of 325 AFLP and 38 RFLP markers, six quantitative trait loci (QTLs) and three unmapped marker loci associated with timing of bud flush were detected. Four QTLs were detected in the field experiment while two QTLs and three unmapped marker loci were identified in the indoor experiment. One QTL associated with indoor bud flushing coincided with one of the QTL detected from the field data. Individual QTL explained 6-16% of the phenotypic variance [corrected]. None of the bud flush QTLs coincided with QTLs controlling height growth identified previously in the same pedigree.
休眠解除是一个重要的物候阶段，它决定了北半球温带地区植物的生长和存活。对一个柳树谱系（n = 82）的春季芽萌发进行了研究，该谱系源自雄性杂交克隆“比约恩”（Salix viminalis x Salix schwerinii）与雌性克隆“78183”（Salix viminalis）的杂交。连续两年（1998年、1999年）在户外记录芽萌发的时间，并于1998年春季在室内记录。发现芽萌发的时间受到中等强度的遗传控制（克隆平均遗传力范围为0.43至0.72）。高度生长与芽萌发之间的表型相关性为负但不显著（r = 0.1 - 0.3）。利用由325个AFLP和38个RFLP标记组成的柳树连锁图谱，检测到6个与芽萌发时间相关的数量性状位点（QTL）和3个未定位的标记位点。在田间试验中检测到4个QTL，而在室内试验中鉴定出2个QTL和3个未定位的标记位点。一个与室内芽萌发相关的QTL与从田间数据中检测到的一个QTL重合。单个QTL解释了6 - 16%的表型变异[校正后]。没有一个芽萌发QTL与先前在同一谱系中鉴定出的控制高度生长的QTL重合。
