Sauer P, Andrew S E, Lassaline M, Gelatt K N, Denis H M
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-0126, USA.
Vet Ophthalmol. 2003 Dec;6(4):309-13. doi: 10.1111/j.1463-5224.2003.00312.x.
To document changes in antibiotic resistance of organisms in cases of equine bacterial ulcerative keratitis over a 10-year time period.
A retrospective study.
Medical records of equine patients with bacterial ulcerative keratitis seen at the University of Florida's VMTH for the years 1991-2000 were reviewed.
All cases of equine bacterial ulcerative keratitis for the above mentioned years were examined. Bacterial isolates were identified and subjected to Kirby-Bauer disc diffusion method sensitivity tests. Antibiotics used in the sensitivity tests included bacitracin, ampicillin, gentamicin, chloramphenicol, polymyxin B, trimethoprim-sulfa, neomycin, kanamycin, carbenicillin, tobramycin and enrofloxacin.
A total of 65 bacterial isolates were subjected to sensitivity testing. Of these isolates, Pseudomonas aeruginosa accounted for 14 of the bacterial isolates (22%), Streptococcus equi subspecies zooepidemicus accounted for 13 of the bacterial isolates (20%), and Staphylococcus aureus accounted for four of the isolates (6%). A statistically significant increase in resistance of Pseudomonas aeruginosa isolates to the antibiotics gentamicin and tobramycin was found between the isolates from 1992 to 1998 and those from 1999 to 2000. An increase in resistance of Streptococcus equi subspecies zooepidemicus to gentamicin was found between the isolates from 1993 to 1997 and those from 1998 to 2000.
Streptococcus equi subspecies zooepidemicus and Pseudomonas aeruginosa were the most common organisms isolated from cases of equine bacterial keratitis referred to the University of Florida's VMTH for the years 1991-2000. There appears to be an increase in resistance of Streptococcus equi subspecies zooepidemicus to gentamicin over the past 10 years. In addition, there is a significant increase in resistance of Pseudomonas aeruginosa to both gentamicin and tobramycin over the same time period.
记录10年间马细菌性溃疡性角膜炎病例中微生物的抗生素耐药性变化。
一项回顾性研究。
回顾了1991年至2000年在佛罗里达大学兽医学院就诊的马细菌性溃疡性角膜炎患者的病历。
检查上述年份所有马细菌性溃疡性角膜炎病例。鉴定细菌分离株，并进行 Kirby-Bauer 纸片扩散法敏感性试验。敏感性试验中使用的抗生素包括杆菌肽、氨苄青霉素、庆大霉素、氯霉素、多粘菌素B、甲氧苄啶-磺胺、新霉素、卡那霉素、羧苄青霉素、妥布霉素和恩诺沙星。
共对65株细菌分离株进行了敏感性试验。其中，铜绿假单胞菌占14株（22%），马链球菌兽疫亚种占13株（20%），金黄色葡萄球菌占4株（6%）。发现1992年至1998年分离株与1999年至2000年分离株相比，铜绿假单胞菌分离株对庆大霉素和妥布霉素的耐药性有统计学显著增加。发现1993年至1997年分离株与1998年至2000年分离株相比，马链球菌兽疫亚种对庆大霉素的耐药性增加。
1991年至2000年转诊至佛罗里达大学兽医学院的马细菌性角膜炎病例中，最常分离出的微生物是马链球菌兽疫亚种和铜绿假单胞菌。在过去10年中，马链球菌兽疫亚种对庆大霉素的耐药性似乎有所增加。此外，在同一时期，铜绿假单胞菌对庆大霉素和妥布霉素的耐药性显著增加。
