Revilla C, Rodríguez-Carreño M P, Alvarez B, Chamorro S, Alonso L M, Ezquerra A, Alonso F, Domínguez J
Departamento de Biotecnología, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ctra de la Coruña, km 7, 28040 Madrid, Spain.
Dev Comp Immunol. 2004 Mar;28(3):239-50. doi: 10.1016/s0145-305x(03)00137-x.
We describe a novel antigen recognized by mAb 2E3 selectively expressed in the periphery by a subset of porcine CD4+ T cells. Both, CD4+CD8alpha- and CD4+CD8alphalow T cell subpopulations express this antigen. CD4+2E3+ T cells show phenotypical and functional characteristics of nai;ve cells. The majority of them are CD29low, CD45RAhigh, CD49dlow, CD11alow, CD18low, and SLA-II-. After mitogen activation CD4+2E3+ T cells express high levels of IL-2 mRNA, but only traces of IFN-gamma or IL-4 mRNA. Indeed a minor percentage of cells stained positive for IFN-gamma when assessed by flow cytometry. Moreover, CD4+2E3+ T cells did not proliferate in response to the recall antigen lysozyme, although they did efficiently to the mitogen ConA. By contrast, CD4+2E3- T cells show phenotypical and functional characteristics of primed cells. They express markers associated to a memory phenotype, respond to the recall antigen lysozyme, and produce high amounts of IFN-gamma and IL-4.
我们描述了一种由单克隆抗体2E3识别的新型抗原，该抗原由猪CD4⁺ T细胞亚群在外周选择性表达。CD4⁺CD8α⁻和CD4⁺CD8α低T细胞亚群均表达这种抗原。CD4⁺2E3⁺ T细胞表现出幼稚细胞的表型和功能特征。它们中的大多数是CD29低、CD45RA高、CD49d低、CD11a低、CD18低和SLA-II⁻。在有丝分裂原激活后，CD4⁺2E3⁺ T细胞表达高水平的IL-2 mRNA，但仅微量的IFN-γ或IL-4 mRNA。实际上，通过流式细胞术评估时，有一小部分细胞IFN-γ染色呈阳性。此外，CD4⁺2E3⁺ T细胞对回忆抗原溶菌酶不发生增殖反应，尽管它们对有丝分裂原ConA反应有效。相比之下，CD4⁺2E3⁻ T细胞表现出已致敏细胞的表型和功能特征。它们表达与记忆表型相关的标志物，对回忆抗原溶菌酶有反应，并产生大量的IFN-γ和IL-4。
