Departamento de Biotecnología, Centro Nacional Instituto de Investigación y Tecnología Agraria y Alimentaria (INIA-CSIC), Madrid, 28040, Spain.
Grupo de Inmunogenómica y Patogénesis Molecular (IMIBIC), Departamento de Genética, Universidad de Córdoba, Campus Universitario de Rabanales, 14014, Córdoba, Spain.
Dev Comp Immunol. 2022 Aug;133:104431. doi: 10.1016/j.dci.2022.104431. Epub 2022 May 5.
In this report, we describe the characterization of a new monoclonal antibody, named 4H5CR4, against porcine CD9. Its use in combination with antibodies to CD4, CD8α, and 2E3 allows to distinguish at least five main CD4 T cell subsets. Analysis on these subsets of CD45RA, CD27, CD29, CD95, CCR7, and SLA-DR markers depicts a progressive model of CD4 T cell development. CD4 2E3 CD8α CD9 cells are the least differentiated population of naïve cells, whereas the CD4 2E3 CD8αCD9 and CD4 2E3 CD8α CD9 cells display phenotypic features of central and effector memory T helper cells, respectively. The latter subsets were able to produce IFN-γ after polyclonal activation with PMA/Ionomycin; however, in vitro virus-specific IFN-γ production of PBMCs collected at 38-44 days after pseudorabies virus vaccination was dominated by cells with a CD9 phenotype. Therefore, CD9 appears to be a useful marker to investigate CD4 T cell heterogeneity in swine.
在本报告中，我们描述了一种针对猪 CD9 的新型单克隆抗体 4H5CR4 的鉴定。它与 CD4、CD8α 和 2E3 抗体联合使用，可以区分至少五个主要的 CD4 T 细胞亚群。对这些 CD45RA、CD27、CD29、CD95、CCR7 和 SLA-DR 标志物的亚群分析描绘了 CD4 T 细胞发育的渐进模型。CD4 2E3 CD8α CD9 细胞是幼稚细胞中分化程度最低的群体，而 CD4 2E3 CD8α CD9 和 CD4 2E3 CD8α CD9 细胞分别显示中央和效应记忆 T 辅助细胞的表型特征。这些亚群在 PMA/离子霉素多克隆激活后能够产生 IFN-γ；然而，在伪狂犬病病毒接种后 38-44 天采集的 PBMC 中，体外病毒特异性 IFN-γ 的产生主要由具有 CD9 表型的细胞主导。因此，CD9 似乎是研究猪 CD4 T 细胞异质性的有用标记物。
