在体内局部应用5-氨基酮戊酸（ALA）和六氢-5-氨基酮戊酸（h-ALA）后，人肿瘤细胞中原卟啉IX的蓄积情况。

Protoporphyrin IX-accumulation in human tumor cells following topical ALA- and h-ALA-application in vivo.

作者信息

Zenzen Volker, Zankl Heinrich

机构信息

Department of Human Biology and Human Genetics, The University, P.O. Box 3049, D-67653 Kaiserslautern, Germany.

出版信息

Cancer Lett. 2003 Dec 8;202(1):35-42. doi: 10.1016/j.canlet.2003.07.001.

DOI:10.1016/j.canlet.2003.07.001

PMID:14643024

Abstract

The fluorescence monitoring represents an innovative approach to detect tumor tissue by photosensitizer-mediated fluorescence. Therefore, information on cellular uptake, tumor selectivity and accumulation properties of photosensitizers are of essential interest. In this study we compared the accumulation properties of two photosensitizer precursors, the 5-aminolaevulinic acid (ALA) and a hexylester of ALA (h-ALA), in vivo using the hen's egg model and the human larynx carcinoma cell line HEp-2. The formation of the actual photosensitizer, protoporphyrin IX (PpIX), was determined both qualitatively and quantitatively. The intensity of the excited PpIX-fluorescence was observed as an indicator for the presence of PpIX after topical ALA- and h-ALA-applications. PpIX-fluorescence was measured using spatially resolved fluorescence spectroscopy.

摘要

荧光监测是一种通过光敏剂介导的荧光来检测肿瘤组织的创新方法。因此，光敏剂的细胞摄取、肿瘤选择性和积累特性的信息至关重要。在本研究中，我们使用鸡蛋模型和人喉癌细胞系HEp-2在体内比较了两种光敏剂前体，即5-氨基乙酰丙酸（ALA）和ALA的己酯（h-ALA）的积累特性。对实际光敏剂原卟啉IX（PpIX）的形成进行了定性和定量测定。在局部应用ALA和h-ALA后，观察激发的PpIX荧光强度作为PpIX存在的指标。使用空间分辨荧光光谱法测量PpIX荧光。