Calin Mihaela Antonina, Gruia Maria luliana, Herascu Nicoleta, Coman Toma
national Institute for Research and Development for Optoelectronics, INOE 2000, P.O. Box MG5, Bucharest, Romania.
J Exp Ther Oncol. 2004 Oct;4(3):247-51.
Photodynamic therapy with protoporphyrin IX induced by delta-aminolevulinic acid (ALA) is mainly applied for the treatment of human superficial skin cancer. In this paper we present our study on photodynamic therapy (PDT) of the implanted Walker tumours using subcutaneous administration of ALA to improve the availability of ALA in the skin. We determined the accumulation and localization of protoporphyrin IX (PpIX) after subcutaneous administration of different concentrations of ALA in a physiological saline solutions, using fluorescence imaging technique. The results obtained indicate that PpIX accumulation depends on the concentration of ALA. The temporal behavior of PpIX fluorescence has shown a clear demarcation of tumoural zone depending on the post-administration time and the administrated concentration of the ALA solution. Further studies are needed to confirm these encouraging results and to define the PDT protocols using subcutaneous administration of ALA solution
由δ-氨基乙酰丙酸（ALA）诱导的原卟啉IX光动力疗法主要用于治疗人类浅表皮肤癌。在本文中，我们展示了关于使用皮下注射ALA来提高皮肤中ALA可用性，对植入的Walker肿瘤进行光动力疗法（PDT）的研究。我们使用荧光成像技术，测定了在生理盐溶液中皮下注射不同浓度ALA后原卟啉IX（PpIX）的积累和定位。所得结果表明，PpIX的积累取决于ALA的浓度。PpIX荧光的时间行为显示，根据给药后的时间和ALA溶液的给药浓度，肿瘤区域有明显的界限。需要进一步研究以证实这些令人鼓舞的结果，并确定使用皮下注射ALA溶液的PDT方案。
