Yingge Zhang, Xia Jiang, Lan Sun
Beijing Institute of Pharmacology and Toxicology, 27 Taiping Road, Beijing 100850, China.
J Struct Biol. 2003 Dec;144(3):327-36. doi: 10.1016/j.jsb.2003.09.022.
The relations between neurite development and the subcellular structures of the hippocampal neuron somata have been studied with atomic force microscopy (AFM). The conformation of the neuron was achieved by the synapse-like structures found by AFM scanning along a neurite of the cell. Hippocampal neuron somata were divided into two or three subcellular parts by one or two horizontal grooves. The upper parts increased while the middle and the lower parts decreased with the number and the length of the neurites and the formation of the neurosynapse-like structures. When neurites sufficiently developed, the middle parts were lost and the lower parts became very small. Mitosis inhibitors could prevent the formation of such subcellular structures of hippocampal neuron somata, which was accompanied by the loss of ability to form synapse-like structures. These results suggest that the upper parts are responsible for neuritogenesis while the middle and the lower parts only have indirect effect on it.
利用原子力显微镜（AFM）研究了神经突发育与海马神经元胞体亚细胞结构之间的关系。通过AFM沿细胞神经突扫描发现的突触样结构实现了神经元的构象。海马神经元胞体被一或两条水平沟分为两或三个亚细胞部分。随着神经突的数量、长度以及神经突触样结构的形成，上部增加，而中部和下部减少。当神经突充分发育时，中部消失，下部变得非常小。有丝分裂抑制剂可阻止海马神经元胞体这种亚细胞结构的形成，同时伴有形成突触样结构能力的丧失。这些结果表明，上部负责神经突发生，而中部和下部仅对其有间接影响。
