利用原子力显微镜对膜蛋白和微丝进行分子成像。
Molecular imaging of membrane proteins and microfilaments using atomic force microscopy.
机构信息
Department of Molecular and Cellular Biochemistry, Kangwon National University School of Medicine, Chuncheon 200-701, Korea.
出版信息
Exp Mol Med. 2010 Sep 30;42(9):597-605. doi: 10.3858/emm.2010.42.9.064.
Abstract
Atomic force microscopy (AFM) is an emerging technique for a variety of uses involving the analysis of cells. AFM is widely applied to obtain information about both cellular structural and subcellular events. In particular, a variety of investigations into membrane proteins and microfilaments were performed with AFM. Here, we introduce applications of AFM to molecular imaging of membrane proteins, and various approaches for observation and identification of intracellular microfilaments at the molecular level. These approaches can contribute to many applications of AFM in cell imaging.
摘要
原子力显微镜（AFM）是一种新兴的技术，可用于多种涉及细胞分析的用途。AFM 广泛应用于获取有关细胞结构和亚细胞事件的信息。特别是，已经使用 AFM 进行了多种膜蛋白和微丝的研究。在这里，我们介绍了 AFM 在膜蛋白分子成像中的应用，以及在分子水平上观察和识别细胞内微丝的各种方法。这些方法可以为 AFM 在细胞成像中的许多应用做出贡献。
相似文献
1
Molecular imaging of membrane proteins and microfilaments using atomic force microscopy.
Exp Mol Med. 2010 Sep 30;42(9):597-605. doi: 10.3858/emm.2010.42.9.064.
2
High-speed atomic force microscopy: imaging and force spectroscopy.
FEBS Lett. 2014 Oct 1;588(19):3631-8. doi: 10.1016/j.febslet.2014.06.028. Epub 2014 Jun 14.
3
Imaging fibroblast cells using atomic force microscopy (AFM).
Cold Spring Harb Protoc. 2010 Oct 1;2010(10):pdb.prot5500. doi: 10.1101/pdb.prot5500.
4
Identification and ultrastructural imaging of photodynamic therapy-induced microfilaments by atomic force microscopy.
Ultramicroscopy. 2009 Nov;109(12):1428-34. doi: 10.1016/j.ultramic.2009.07.009. Epub 2009 Jul 24.
5
Cytoskeleton induced the changes of microvilli and mechanical properties in living cells by atomic force microscopy.
J Cell Physiol. 2021 May;236(5):3725-3733. doi: 10.1002/jcp.30110. Epub 2020 Nov 10.
6
Imaging and interrogating native membrane proteins using the atomic force microscope.
Methods Mol Biol. 2011;736:153-67. doi: 10.1007/978-1-61779-105-5_11.
7
Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy.
Methods Mol Biol. 2011;736:47-60. doi: 10.1007/978-1-61779-105-5_4.
8
Nanoscale imaging and force probing of biomolecular systems using atomic force microscopy: from single molecules to living cells.
Nanoscale. 2017 Nov 23;9(45):17643-17666. doi: 10.1039/c7nr07023c.
9
High-speed atomic force microscopy: Structure and dynamics of single proteins.
Curr Opin Chem Biol. 2011 Oct;15(5):704-9. doi: 10.1016/j.cbpa.2011.05.007. Epub 2011 May 31.
10
Use of the unroofing technique for atomic force microscopic imaging of the intra-cellular cytoskeleton under aqueous conditions.
J Electron Microsc (Tokyo). 2012;61(5):321-6. doi: 10.1093/jmicro/dfs055. Epub 2012 Aug 7.
引用本文的文献
1
Applying the Atomic Force Microscopy Technique in Medical Sciences-A Narrative Review.
Biomedicines. 2024 Sep 3;12(9):2012. doi: 10.3390/biomedicines12092012.
2
Analytical Model for Determination of Size-Distribution of Colloidal Silver Nanoparticles from Surface Plasmon Resonance Wavelength and Dielectric Functions.
Nanomaterials (Basel). 2022 Oct 4;12(19):3474. doi: 10.3390/nano12193474.
3
Targeting cell-matrix interface mechanobiology by integrating AFM with fluorescence microscopy.
Prog Biophys Mol Biol. 2022 Dec;176:67-81. doi: 10.1016/j.pbiomolbio.2022.08.005. Epub 2022 Aug 30.
4
Understanding nanoparticle endocytosis to improve targeting strategies in nanomedicine.
Chem Soc Rev. 2021 May 7;50(9):5397-5434. doi: 10.1039/d0cs01127d. Epub 2021 Mar 5.
5
Direct imaging of antigen-antibody binding by atomic force microscopy.
Appl Nanosci. 2021;11(1):293-300. doi: 10.1007/s13204-020-01558-w. Epub 2020 Sep 24.
6
Nano-scientific Application of Atomic Force Microscopy in Pathology: from Molecules to Tissues.
Int J Med Sci. 2020 Mar 15;17(7):844-858. doi: 10.7150/ijms.41805. eCollection 2020.
7
Investigating cell mechanics with atomic force microscopy.
J R Soc Interface. 2015 Mar 6;12(104):20140970. doi: 10.1098/rsif.2014.0970.
8
Measurement of cationic and intracellular modulation of integrin binding affinity by AFM-based nanorobot.
Biophys J. 2013 Jul 2;105(1):40-7. doi: 10.1016/j.bpj.2013.05.052.
9
Dysferlin-peptides reallocate mutated dysferlin thereby restoring function.
PLoS One. 2012;7(11):e49603. doi: 10.1371/journal.pone.0049603. Epub 2012 Nov 20.
本文引用的文献
1
Demonstration of a direct interaction between sigma-1 receptors and acid-sensing ion channels.
Biophys J. 2010 Apr 7;98(7):1182-91. doi: 10.1016/j.bpj.2009.12.4293.
2
AFM observation of single, functioning ionotropic glutamate receptors reconstituted in lipid bilayers.
Biochim Biophys Acta. 2010 Jul;1800(7):655-61. doi: 10.1016/j.bbagen.2010.03.002. Epub 2010 Mar 20.
3
AFM imaging reveals the tetrameric structure of the TRPM8 channel.
Biochem Biophys Res Commun. 2010 Apr 2;394(2):383-6. doi: 10.1016/j.bbrc.2010.03.027. Epub 2010 Mar 7.
4
Improved localization of cellular membrane receptors using combined fluorescence microscopy and simultaneous topography and recognition imaging.
Nanotechnology. 2010 Mar 19;21(11):115504. doi: 10.1088/0957-4484/21/11/115504. Epub 2010 Feb 22.
5
Cell mechanics and the cytoskeleton.
Nature. 2010 Jan 28;463(7280):485-92. doi: 10.1038/nature08908.
6
Molecular shape and binding force of Mycoplasma mobile's leg protein Gli349 revealed by an AFM study.
Biochem Biophys Res Commun. 2010 Jan 15;391(3):1312-7. doi: 10.1016/j.bbrc.2009.12.023. Epub 2009 Dec 14.
7
Localization of Na+-K+ ATPases in quasi-native cell membranes.
Nano Lett. 2009 Dec;9(12):4489-93. doi: 10.1021/nl902803m.
8
Single-molecule dynamics of the DNA-EcoRII protein complexes revealed with high-speed atomic force microscopy.
Biochemistry. 2009 Nov 10;48(44):10492-8. doi: 10.1021/bi9010368.
9
Antitumor effect of photodynamic therapy with chlorin-based photosensitizer DH-II-24 in colorectal carcinoma.
Cancer Sci. 2009 Dec;100(12):2431-6. doi: 10.1111/j.1349-7006.2009.01326.x. Epub 2009 Aug 25.
10
Structural plasticity in actin and tubulin polymer dynamics.
Science. 2009 Aug 21;325(5943):960-3. doi: 10.1126/science.1168823.
Zotero 插件