Choich J A, El-Nabawi A, Silbergeld E K
Department of Toxicology, University of Maryland at Baltimore, Baltimore, MD, USA.
Environ Res. 2004 Jan;94(1):86-93. doi: 10.1016/s0013-9351(03)00111-7.
It was hypothesized that fish possess functioning H1 histamine receptors that have the ability to bind agonists and antagonists specific to the H1 histamine receptor subtype. For these experiments, a combination of a novel, in vivo 2-deoxyglucose method and a standard in vitro autoradiography procedure was utilized. A regional, statistically significant dose response in neurological functioning was observed when fish were exposed to histaminergic agents (i.e., H1 agonists and antagonists), which created the first neurological profile for the H1 histamine receptor in fish brain. The H1 histamine receptor was chosen as a characterization receptor in fish because histamine has been linked to a variety of neurological functions such as the control of arousal, attention, sensory processing, and cognition. Histamine also plays a role in pituitary hormone secretion, appetite control, and, potentially, regulation of vestigular reactivity. In addition, the fish brain is well characterized structurally, and the existence of an H3-like receptor has been documented recently in zebrafish. However, to date there is little detailed information about specific localization and functioning of the H1 histamine receptor in fish.
据推测，鱼类拥有可正常运作的H1组胺受体，该受体能够结合H1组胺受体亚型特异性的激动剂和拮抗剂。在这些实验中，采用了一种新型的体内2-脱氧葡萄糖方法与标准的体外放射自显影程序相结合的方式。当鱼类暴露于组胺能药物（即H1激动剂和拮抗剂）时，观察到神经功能出现了具有统计学意义的区域剂量反应，这为鱼类脑中的H1组胺受体建立了首个神经学特征。选择H1组胺受体作为鱼类的特征性受体是因为组胺与多种神经功能相关，如觉醒、注意力、感觉处理和认知的控制。组胺在垂体激素分泌、食欲控制以及可能的前庭反应调节中也发挥作用。此外，鱼类大脑在结构上有很好的特征描述，并且最近在斑马鱼中已记录到存在类似H3的受体。然而，迄今为止，关于鱼类中H1组胺受体的具体定位和功能几乎没有详细信息。
