Wang Wen, Rong Liwen, Gu Wei, Du Kaihe, Chen Jianxiu
College of Biological Sciences, Nanjing Normal University, 210097, Nanjing, PR China.
Res Microbiol. 2003 Dec;154(10):677-80. doi: 10.1016/j.resmic.2003.08.004.
Tremor disease (TD) of the Chinese mitten crab Eriocheir sinensi has become a serious disease in the Chinese freshwater culture industry in recent years. The agent, belonging to Spiroplasma, was purified from yolk and allantoic fluids of chicken embryos and was then inoculated into the body of crayfish, the abdominal cavity of ICR mice and the allantoid of chicken embryos, respectively. Thirty-eight days after the inoculation, the mice and crayfish were dissected and their tissues sampled and observed with both the light and electron microscope. No infection was detected in mouse or crawfish tissue. But when the different tissues from the inoculated embryonated chickens were tested, the agent was detected only in the brain of embryonated chickens. This indicated that the agent represented a neurotropic characteristic in embryonated chickens, just as it had in the crab. This infective characteristic may be due to the development and maturation of host immunity.
近年来，中华绒螯蟹颤抖病已成为中国淡水养殖业中的一种严重疾病。该病原体属于螺原体，从鸡胚的卵黄和尿囊液中纯化后，分别接种到小龙虾体内、ICR小鼠腹腔和鸡胚尿囊。接种38天后，解剖小鼠和小龙虾，采集其组织，并用光学显微镜和电子显微镜进行观察。在小鼠或小龙虾组织中未检测到感染。但是，对接种后的胚胎鸡的不同组织进行检测时，仅在胚胎鸡的脑中检测到该病原体。这表明该病原体在胚胎鸡中表现出嗜神经性特征，就像在蟹中一样。这种感染特性可能归因于宿主免疫力的发育和成熟。
