Zilly Michael, Langmann Peter, Lenker Ulrike, Satzinger Verena, Schirmer Diana, Klinker Hartwig
Department of Internal Medicine, Division of Infectious Diseases and Hepatology, Medizinische Poliklinik, University of Wuerzburg, Josef-Schneider Strasse 2, D-97080, Wuerzburg, Germany.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Dec 25;798(2):179-86. doi: 10.1016/j.jchromb.2003.09.037.
In order to evaluate recent alcohol consumption, a very sensitive and specific gas chromatographic method for ethanol determination in human urine samples was developed. The non-invasive method was performed without any pretreatment and carried out on a Stabilwax capillary column, 30 m x 0.53 mm x 1.0 microm film thickness. Helium was used as carrier gas with a constant inlet pressure of 27.72 kPa (0.277 bar) and a flame ionization detector (FID). Quantification was performed with the use of acetonitrile as an internal standard (IS). The calibration curve was linear throughout the concentration range from 0.5 to 500 mg/l. The calculated intra- and inter-day coefficients of variation were below 8%. A clear chromatographic separation of ethanol from methanol, acetone, 1-propanol and 2-propanol was achieved.
为了评估近期的酒精摄入量，开发了一种用于测定人尿液样本中乙醇的非常灵敏且特异的气相色谱法。该非侵入性方法无需任何预处理，在一根30 m×0.53 mm×1.0微米膜厚的Stabilwax毛细管柱上进行。以氦气作为载气，恒定入口压力为27.72 kPa（0.277 bar），并使用火焰离子化检测器（FID）。使用乙腈作为内标（IS）进行定量。校准曲线在0.5至500 mg/l的浓度范围内呈线性。计算得出的日内和日间变异系数均低于8%。实现了乙醇与甲醇、丙酮、1-丙醇和2-丙醇的清晰色谱分离。
