Sirianuntapiboon Suntud, Phothilangka Phimphaka, Ohmomo Sadahiro
Department of Environmental Technology, School of Energy and Materials, King Mongkut's University of Technology Thonburi (KMUTT), Bangkok 10140, Thailand.
Bioresour Technol. 2004 Mar;92(1):31-9. doi: 10.1016/j.biortech.2003.07.010.
Among 170 strains of acetogenic bacteria, a strain No.BP103 showed the highest decolorization yield (76.4+/-3.2%) when cultivated at 30 degrees C for 5 days in molasses pigments medium containing glucose 3.0%, yeast extract 0.5%, KH2PO4 0.1% and MgSO4 x 7H2O 0.05% and the pH adjusted to 6.0. In addition this strain could decolorize 32.3+/-3.2% and 73.5+/-3.5% of molasses pigments in stillage and anaerobically treated molasses wastewater, both supplementing glucose 3.0%, yeast extract 0.5%, KH2PO4 0.1% and MgSO4 x 7H2O 0.05%. However without nutrients supplement, the decolorization yields were 9.75+/-3.0% and 44.36+/-3.4%, respectively. In a replacement culture system involving six times replacement (30 days), the strain No.BP103 showed a constant decolorization yield of 72.0+/-3.2-84.0+/-3.2% and caused decreases of biological oxygen demand and chemical oxygen demand values of approximately 58.5+/-6.4-82.2+/-7.1% and 35.5+/-0.42-71.20.58%, respectively. Under a periodical feeding system, the decolorization yield was 30.0+/-2.1-45.0+/-3.5%.
在170株产乙酸细菌中,一株编号为BP103的菌株在含有3.0%葡萄糖、0.5%酵母提取物、0.1% KH₂PO₄和0.05% MgSO₄·7H₂O且pH值调至6.0的糖蜜色素培养基中于30℃培养5天时,表现出最高的脱色率(76.4±3.2%)。此外,该菌株能够使含有3.0%葡萄糖、0.5%酵母提取物、0.1% KH₂PO₄和0.05% MgSO₄·7H₂O的酒糟滤液和厌氧处理后的糖蜜废水中32.3±3.2%和73.5±3.5%的糖蜜色素脱色。然而,在不补充营养物质的情况下,脱色率分别为9.75±3.0%和44.36±3.4%。在一个涉及六次更换(30天)的替代培养系统中,BP103菌株表现出72.0±3.2 - 84.0±3.2%的恒定脱色率,并使生物需氧量和化学需氧量值分别降低了约58.5±6.4 - 82.2±7.1%和35.5±0.42 - 71.2±0.58%。在定期进料系统下,脱色率为30.0±2.1 - 45.0±3.5%。