发酵性乳酸菌对糖蜜焦糖素和棕榈油厂废水酚类化合物的脱色作用。
Decolorization of molasses melanoidins and palm oil mill effluent phenolic compounds by fermentative lactic acid bacteria.
机构信息
Division of Environmental Technology, School of Energy, Environment and Materials, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
出版信息
J Environ Sci (China). 2010;22(8):1209-17. doi: 10.1016/s1001-0742(09)60240-0.
Lactobacillus plantarum SF5.6 is one of the lactic acid bacteria (LAB) that has the highest ability of molasses melanoidin (MM) decolorization among the 2114 strains of LAB. The strains were isolated from spoilage, pickle fruit and vegetable, soil and sludge from the wastewater treatment system by using technical step of enrichment, primary screening and secondary screening. This LAB strain SF5.6 was identified by 16S rDNA analysis and carbohydrate fermentation (API 50 CH). The top five LAB strains having high MM decolorization (> 55%), namely TBSF5.8-1, TBSF2.1-1, TBSF2.1, FF4A and SF5.6 were selected to determine the optimal condition. It was found that the temperature at 30 degrees C under facultative conditions in GPY-MM medium (0.5% glucose, 0.1% peptone, 0.1% yeast extract, 0.1% sodium acetate, 0.05% MgSO4 and 0.005% MnCl2 in MM solution at pH 6) giving a high microbial growth and MM decolorization for all five strains. It was noticed that the decolorization of MM by LAB strains might be cell growth associated. L. plantarum SF5.6 grew rapidly within one day while the other strains took 2-3 days. This L. plantarum SF5.6 could rapidly decolorize MM to 60.91% without any lag phase, and it also had the ability to remove 34.00% phenolic compounds and 15.88% color from treated palm oil mill effluent.
植物乳杆菌 SF5.6 是 2114 株乳酸菌（LAB）中具有最高糖蜜类黑精（MM）脱色能力的 LAB 之一。这些菌株是通过富集、初步筛选和二次筛选等技术步骤，从腐败、腌制水果和蔬菜、土壤和污水处理系统的污泥中分离出来的。该 LAB 菌株 SF5.6 通过 16S rDNA 分析和碳水化合物发酵（API 50 CH）进行鉴定。选择了 5 株具有高 MM 脱色能力（>55%）的 LAB 菌株，即 TBSF5.8-1、TBSF2.1-1、TBSF2.1、FF4A 和 SF5.6，以确定最佳条件。结果发现，在 GPY-MM 培养基（MM 溶液中的 0.5%葡萄糖、0.1%蛋白胨、0.1%酵母提取物、0.1%乙酸钠、0.05%MgSO4 和 0.005%MnCl2，pH6）中，在兼性条件下，温度为 30°C 时，所有 5 株菌的微生物生长和 MM 脱色效果最佳。注意到 LAB 菌株对 MM 的脱色可能与细胞生长有关。植物乳杆菌 SF5.6 在一天内迅速生长，而其他菌株需要 2-3 天。该植物乳杆菌 SF5.6 能够快速将 MM 脱色至 60.91%，无需任何迟滞期，还能够去除处理棕榈油厂废水 34.00%的酚类化合物和 15.88%的颜色。
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