Kurbanoglu Esabi Basaran
Department of Biology, Science and Letters Faculty, Atatürk University, 25240 Erzurum, Turkey.
Bioresour Technol. 2004 Mar;92(1):97-101. doi: 10.1016/j.biortech.2003.08.002.
The potential use of ram horn hydrolysate (RHH) as a supplement for improvement of citric acid production by Aspergillus niger NRRL 330 was studied. For this purpose, first RHH was produced. Ram horns were hydrolyzed by treating with acid (6 N-H2SO4) and the RHH was obtained. With the addition of RHH to the fermentation medium with a final concentration of 4% (optimal concentration), citric acid value reached a maximum value (94 g/l), which is 52% higher than that of the control experiment. The addition of 4% (v/v) RHH enhanced citric acid accumulation, reduced residual sugar concentration and stimulated mycelial growth. Adding 4% RHH had no adverse effects on A. niger. As a result, RHH was found to be suitable as a valuable supplement for citric acid production in the submerged fermentation.
研究了公羊角水解物（RHH）作为补充剂用于提高黑曲霉NRRL 330产柠檬酸的潜在用途。为此，首先制备了RHH。通过用酸（6N - H₂SO₄）处理水解公羊角，得到了RHH。在发酵培养基中添加终浓度为4%（最佳浓度）的RHH时，柠檬酸值达到最大值（94 g/l），比对照实验高52%。添加4%（v/v）的RHH可增强柠檬酸积累，降低残余糖浓度并刺激菌丝体生长。添加4%的RHH对黑曲霉没有不良影响。结果表明，RHH适合作为深层发酵生产柠檬酸的有价值补充剂。
