Lin Pinpin, Chang Han, Ho William L, Wu Meng-Hsiang, Su Jang-Ming
Institute of Toxicology, Chung-Shan Medical University, No. 110, Sec 1, Chien-Kuo N. Rd., Taichung 402, Taiwan, ROC.
Lung Cancer. 2003 Dec;42(3):255-61. doi: 10.1016/s0169-5002(03)00359-3.
It has been shown that cytochrome P4501B1 (CYP1B1) is over-expressed in variety of tumors. The objective of this study was to examine the expression and localization of CYP1B1 in normal lungs and tumor tissues of non-small cell lung cancers (NSCLC). CYP1B1 was detectable in the microsomes of normal lungs and lung tumors with Western immunoblotting. Utilizing immunohistochemical staining, we found that CYP1B1 was constitutively expressed in the cytoplasm of smooth muscle cells, but not in pneumocytes. Bronchiolar epithelia from three out of 19 normal lungs expressed CYP1B1. Among 89 NSCLC tumor tissues, more than 50% expressed CYP1B1. In addition, CYP1B1 expression was more common in adenocarcinomas than in squamous cell carcinomas. Recently, we reported over-expression of aryl hydrocarbon receptors (AhR) in lung adenocarcinomas. In the present study, we observed an association between CYP1B1 and AhR expression in 89 NSCLC tumor tissues. These results suggest that CYP1B1 overexpression in NSCLC is accompanied by up-regulation of AhR expression.
研究表明，细胞色素P4501B1（CYP1B1）在多种肿瘤中过度表达。本研究的目的是检测CYP1B1在非小细胞肺癌（NSCLC）正常肺组织和肿瘤组织中的表达及定位。通过蛋白质免疫印迹法在正常肺组织和肺肿瘤的微粒体中检测到CYP1B1。利用免疫组织化学染色，我们发现CYP1B1在平滑肌细胞的细胞质中组成性表达，但在肺细胞中不表达。19例正常肺组织中有3例的细支气管上皮表达CYP1B1。在89例NSCLC肿瘤组织中，超过50%表达CYP1B1。此外，CYP1B1在腺癌中的表达比在鳞状细胞癌中更常见。最近，我们报道了肺腺癌中芳烃受体（AhR）的过度表达。在本研究中，我们观察到89例NSCLC肿瘤组织中CYP1B1与AhR表达之间存在关联。这些结果表明，NSCLC中CYP1B1的过度表达伴随着AhR表达的上调。
