用于tRNA摆动位置修饰尿苷残基的新型甲基转移酶。
Novel methyltransferase for modified uridine residues at the wobble position of tRNA.
作者信息
Kalhor Hamid R, Clarke Steven
机构信息
Department of Chemistry and Biochemistry, UCLA Molecular Biology Institute, University of California-Los Angeles, 611 Charles E. Young Drive East, Los Angeles, CA 90095, USA.
出版信息
Mol Cell Biol. 2003 Dec;23(24):9283-92. doi: 10.1128/MCB.23.24.9283-9292.2003.
Abstract
We have identified a novel tRNA methyltransferase in Saccharomyces cerevisiae that we designate Trm9. This enzyme, the product of the YML014w gene, catalyzes the esterification of modified uridine nucleotides, resulting in the formation of 5-methylcarbonylmethyluridine in tRNA(Arg3) and 5-methylcarbonylmethyl-2-thiouridine in tRNA(Glu). In intact yeast cells, disruption of the TRM9 gene results in the complete loss of these modified wobble bases and increased sensitivity at 37 degrees C to paromomycin, a translational inhibitor. These results suggest a role for this potentially reversible methyl esterification reaction when cells are under stress.
摘要
我们在酿酒酵母中鉴定出一种新型的tRNA甲基转移酶，我们将其命名为Trm9。这种酶是YML014w基因的产物，催化修饰的尿苷核苷酸的酯化反应，导致在tRNA(Arg3)中形成5-甲基羰基甲基尿苷，在tRNA(Glu)中形成5-甲基羰基甲基-2-硫尿苷。在完整的酵母细胞中，TRM9基因的破坏导致这些修饰的摆动碱基完全丧失，并在37摄氏度时对翻译抑制剂巴龙霉素的敏感性增加。这些结果表明，当细胞处于应激状态时，这种潜在的可逆甲基酯化反应具有一定作用。
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