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耻垢分枝杆菌Dps的结晶及初步X射线衍射分析

Crystallization and preliminary X-ray diffraction analysis of Mycobacterium smegmatis Dps.

作者信息

Roy Siddhartha, Gupta Surbhi, Das Satyabrata, Sekar K, Chatterji Dipankar, Vijayan M

机构信息

Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.

出版信息

Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2254-6. doi: 10.1107/s0907444903018742. Epub 2003 Nov 27.

DOI:10.1107/s0907444903018742
PMID:14646086
Abstract

Three crystal forms of a DNA-binding protein from stationary phase cells (Dps) of Mycobacterium smegmatis have been grown. They are hexagonal, tetragonal and cubic. The structure of the cubic form, which has one subunit in the asymmetric unit, has been solved using the molecular-replacement method. The application of the crystallographic symmetry operations on the subunit leads to eight dodecamers with 23 symmetry in the unit cell.

摘要

耻垢分枝杆菌稳定期细胞的一种DNA结合蛋白(Dps)已培养出三种晶体形式。它们分别是六方晶型、四方晶型和立方晶型。立方晶型在不对称单元中有一个亚基,其结构已通过分子置换法解析出来。对该亚基应用晶体学对称操作可在晶胞中得到八个具有23对称性的十二聚体。

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