Arif S M, Sang P B, Varshney U, Vijayan M
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore 560 012, India.
Acta Crystallogr F Struct Biol Commun. 2014 Feb;70(Pt 2):190-2. doi: 10.1107/S2053230X13033906. Epub 2014 Jan 21.
Crystallization of MutT2, MSMEG_5148 from Mycobacterium smegmatis, has been carried out and the crystals have been characterized using X-ray diffraction. Matthews coefficient calculation suggests the possibility of one protein molecule in the asymmetric unit of the orthorhombic unit cell, space group P2(1)2(1)2 or P2(1)22. Solution of the structure of the protein by molecular replacement using the known three-dimensional structure of a bacterial Nudix hydrolase is envisaged.
耻垢分枝杆菌的MutT2（MSMEG_5148）已实现结晶，并利用X射线衍射对晶体进行了表征。马修斯系数计算表明，在正交晶胞的不对称单元中可能存在一个蛋白质分子，空间群为P2(1)2(1)2或P2(1)22。设想通过使用细菌Nudix水解酶的已知三维结构，通过分子置换来解析该蛋白质的结构。
