Horigome Daisuke, Satoh Hiroyuki, Uchida Akira
Department of Biomolecular Science, Toho University, 2-2-1 Miyama, Funabashi, Chiba 274-8510, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Dec;59(Pt 12):2283-5. doi: 10.1107/s0907444903019127. Epub 2003 Nov 27.
A water-soluble chlorophyll protein (WSCP) with a chlorophyll a:b ratio of 6:1 from Brassica oleracea L. var. acephala (kale) was purified and crystallized by the hanging-drop vapour-diffusion method using PEG 8000 and zinc acetate as precipitants. The crystal belongs to the hexagonal space group P6(4)22, with unit-cell parameters a = b = 162.2, c = 38.7 A. A native data set was collected to 2.80 A resolution at 293 K using Cu Kalpha radiation from a rotating-anode generator. Preliminary analysis via molecular replacement identified one kale WSCP monomer in the asymmetric unit. The crystal packing showed a tetrameric structure for kale WSCP, as suggested by previous biochemical studies of WSCPs from Brassicaceae plants.
从羽衣甘蓝（Brassica oleracea L. var. acephala）中纯化出一种叶绿素a:b比例为6:1的水溶性叶绿素蛋白（WSCP），并采用悬滴气相扩散法，以聚乙二醇8000和醋酸锌作为沉淀剂进行结晶。该晶体属于六方空间群P6(4)22，晶胞参数a = b = 162.2，c = 38.7 Å。在293 K下，使用旋转阳极发生器产生的Cu Kα辐射收集了分辨率为2.80 Å的天然数据集。通过分子置换的初步分析确定了不对称单元中有一个羽衣甘蓝WSCP单体。如先前对十字花科植物WSCP的生化研究所表明的，羽衣甘蓝WSCP的晶体堆积显示出四聚体结构。
