Ohtsuki Takayuki, Ohshima Shigeru, Uchida Akira
Department of Bimolecular Science, Faculty of Science, Toho University, Miyama 2-2-1, Funabashi, Chiba 274-8510, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt 9):740-1. doi: 10.1107/S1744309107035658. Epub 2007 Aug 10.
A water-soluble chlorophyll-binding protein (WSCP) with photoconvertibility from Chenopodium album was extracted, purified and crystallized in a darkroom. Green crystals suitable for data collection appeared in about 10 d. A native data set was collected to 2.0 A resolution at 100 K. The space group of the crystal was determined to be orthorhombic I222 or I2(1)2(1)2(1), with unit-cell parameters a = 48.13, b = 60.59, c = 107.21 A. Preliminary analysis of the X-ray data indicated that there is one molecule per asymmetric unit.
从藜中提取、纯化并在暗室中结晶出一种具有光转换性的水溶性叶绿素结合蛋白（WSCP）。约10天后出现了适合数据收集的绿色晶体。在100 K下收集到分辨率为2.0 Å的天然数据集。晶体的空间群被确定为正交晶系I222或I2(1)2(1)2(1)，晶胞参数a = 48.13，b = 60.59，c = 107.21 Å。X射线数据的初步分析表明，每个不对称单元中有一个分子。
