Adachi Hiroaki, Takano Kazufumi, Matsumura Hiroyoshi, Inoue Tsuyoshi, Mori Yusuke, Sasaki Takatomo
Department of Electrical Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Synchrotron Radiat. 2004 Jan 1;11(Pt 1):121-4. doi: 10.1107/s0909049503026980. Epub 2003 Nov 28.
We developed two novel methods for growing large, high-quality protein crystals. A two-liquid system enables the convenient extraction of protein crystals without causing mechanical damage due to growth at the interface between two liquids. Since this system does not require limitations on solution volume, it is also suitable for the seed technique, and for growing large crystals. Another new concept is the mild stirring of the solution using the Floating And Stirring Technique (FAST) and the Micro-stirring technique. When compared to conventional techniques, both techniques result in a reduced number of crystals, as well as the growth of large crystals.
我们开发了两种用于生长大型高质量蛋白质晶体的新方法。双液体系能够方便地提取蛋白质晶体，且不会因在两种液体界面处生长而造成机械损伤。由于该体系对溶液体积没有限制，它也适用于晶种技术以及生长大晶体。另一个新概念是使用浮动搅拌技术（FAST）和微搅拌技术对溶液进行温和搅拌。与传统技术相比，这两种技术都能减少晶体数量，并促进大晶体的生长。
