Adachi Hiroaki, Takano Kazufumi, Morikawa Masaaki, Kanaya Shigenori, Yoshimura Masashi, Mori Yusuke, Sasaki Takatomo
Department of Electrical Engineering, Graduate School of Engineering and Venture Business Laboratory, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Acta Crystallogr D Biol Crystallogr. 2003 Jan;59(Pt 1):194-6. doi: 10.1107/s0907444902019741. Epub 2002 Dec 19.
A new method of protein crystallization, the floating-drop vapour-diffusion technique, has been developed. The method combines the traditional sitting-drop vapour-diffusion technique and a novel two-liquid system. A crystallization drop composed of a mixture of sample and reagent floats on an insoluble and very dense liquid. Protein crystals are grown by vapour diffusion at the interface of the two liquids. The method makes it possible to remove the crystals easily without causing mechanical damage. This approach also significantly reduces the time and cost compared with the hanging-drop technique, which is presently the most popular method for protein crystallization.
一种新的蛋白质结晶方法——浮滴气相扩散技术已经被开发出来。该方法结合了传统的坐滴气相扩散技术和一种新型的双液体系。由样品和试剂混合物组成的结晶滴漂浮在一种不溶性且非常致密的液体上。蛋白质晶体通过在两种液体的界面处进行气相扩散而生长。该方法使得能够轻松地移除晶体而不会造成机械损伤。与目前最流行的蛋白质结晶方法——悬滴技术相比，这种方法还显著减少了时间和成本。
