Hirai Nobuhiro, Kondo Satoru, Ohigashi Hajime
International Innovation Center, Kyoto University, Japan.
Biosci Biotechnol Biochem. 2003 Nov;67(11):2408-15. doi: 10.1271/bbb.67.2408.
The concentration of abscisic acid in plants is regulated not only by biosynthesis, but also by metabolism. Abscisic acid is metabolized to phaseic acid via 8'-hydroxyabscisic acid, and phaseic acid is then converted to dihydrophaseic acid and its epimer. A quantitative analysis of these metabolites is important as well as that of abscisic acid to understand changes in the concentration of abscisic acid in plants. However, no internal standards of the metabolites suitable for quantitative analysis have been reported. We prepared 7'-deuterium-labeled phaseic acid with a deuterium content of 86%, using the equilibrium reaction between phaseic acid and 8'-hydroxyabscisic acid. 7'-Deuterium-labeled dihydrophaseic acids were obtained by reducing 7'-deuterium-labeled phaseic acid. The levels of the metabolites in plant organs were determined by using the deuterated metabolites as internal standards.
植物中脱落酸的浓度不仅受生物合成调控，还受代谢调控。脱落酸通过8'-羟基脱落酸代谢为相酸，然后相酸转化为二氢相酸及其差向异构体。对这些代谢物进行定量分析对于理解植物中脱落酸浓度的变化以及对脱落酸进行定量分析都很重要。然而，尚未报道适用于定量分析的这些代谢物的内标。我们利用相酸与8'-羟基脱落酸之间的平衡反应制备了氘含量为86%的7'-氘代相酸。通过还原7'-氘代相酸获得了7'-氘代二氢相酸。以氘代代谢物作为内标物测定了植物器官中代谢物的含量。
