腹腔内补充褪黑素对甲状腺功能亢进大鼠甲状腺激素和睾酮释放的影响。

The effect of intraperitoneal melatonin supplementation on the release of thyroid hormones and testosterone in rats with hyperthyroid.

作者信息

Mogulkoc Rasim, Baltaci Abdulkerim Kasim

机构信息

Selcuk University, Meram Medical School, Department of Physiology, 42080 Konya-Turkey.

出版信息

Neuro Endocrinol Lett. 2003 Oct;24(5):345-7.

PMID:14647010

Abstract

OBJECTIVE

Melatonin has a general inhibitory effect on the reproductive system and thyroid functions. Testosterone secretion increases in parallel to thyroid hormones in hyperthyroidism. The aim of this study was to investigate the effects of melatonin application on total T3, T4 and testosterone in hyperthyroid rats.

METHODS

This study was performed on 4 groups each consisted of 6 male Spraque Dawley rats. Control group (G1); sham group (G2); applied 0.5 ml/day of serum physiologic intraperitoneally for 2 weeks, hyperthyroid group (G3); applied 0.3 mg/kg/day thyroxine intraperitoneally for 2 weeks, and hyperthyroid and melatonin supplemented group (G4); applied 0.3 mg/kg/day thyroxine and 3 mg/kg/day melatonin. Blood samples taken by way of decapitation were analysed for total T3, T4 and testosterone by RIA.

RESULTS

Serum total T3 and total testosterone levels were the highest in hyperthyroid group (G3), whereas in hyperthyroid and melatonin supplemented group (G4) were the lowest (P<0.001). Total T4 levels were higher in group 3 than group 1, 2 and 4 (P<0.001).

CONCLUSION

Results revealed that melatonin supplementation in hyperthyroidism suppress secretion of thyroid hormones and testosterone secretion.

摘要

目的

褪黑素对生殖系统和甲状腺功能具有普遍的抑制作用。甲状腺功能亢进时，睾酮分泌与甲状腺激素平行增加。本研究旨在探讨褪黑素对甲状腺功能亢进大鼠总三碘甲状腺原氨酸（T3）、总甲状腺素（T4）和睾酮的影响。

方法

本研究对4组雄性Spraque Dawley大鼠进行，每组6只。对照组（G1）；假手术组（G2），腹腔注射0.5 ml/天的生理盐水，持续2周；甲状腺功能亢进组（G3），腹腔注射0.3 mg/kg/天的甲状腺素，持续2周；甲状腺功能亢进并补充褪黑素组（G4），腹腔注射0.3 mg/kg/天的甲状腺素和3 mg/kg/天的褪黑素。通过断头采集血样，采用放射免疫分析法分析总T3、T4和睾酮。