Abu-Ghazaleh R I, Dunnette S L, Loegering D A, Checkel J L, Kita H, Thomas L L, Gleich G J
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota 55905.
J Leukoc Biol. 1992 Dec;52(6):611-8. doi: 10.1002/jlb.52.6.611.
Eosinophils contain four principal cationic proteins, major basic protein (MBP), eosinophil-derived neurotoxin (EDN), eosinophil cationic protein (ECP), and eosinophil peroxidase (EPO). To determine the quantities of these proteins in granulocytes and whether they are specific to eosinophils, their concentrations in lysates of human granulocytes were measured using specific radioimmunoassays. The effect of different methods for eosinophil lysis on the recovery of the proteins was also studied. Maximal recovery occurred at pH 2 for MBP and pH 5.6 for the other granule proteins. The proteins cosedimented with eosinophils and their concentrations (mean +/- SEM) in ng/10(6) eosinophils (and in nM/10(6) eosinophils) were: MBP, 8,982 +/- 611 (641.6); EDN, 3,283 +/- 116 (178.4); ECP, 5,269 +/- 283 (250.9); and EPO, 12,174 +/- 859 (171.5). Basophils from a normal person contained (in ng/10(6) cells) MBP, 2,374; EDN, 214; ECP, 77; and EPO, 17. Highly purified neutrophils contained (in ng/10(6) cells) MBP, 3 +/- 0.5; EDN, 72 +/- 9; and ECP, 50 +/- 12. Therefore we conclude that these proteins are mainly expressed in eosinophils, but that certain ones are present in basophils and neutrophils.
嗜酸性粒细胞含有四种主要的阳离子蛋白，即主要碱性蛋白（MBP）、嗜酸性粒细胞衍生神经毒素（EDN）、嗜酸性粒细胞阳离子蛋白（ECP）和嗜酸性粒细胞过氧化物酶（EPO）。为了确定这些蛋白在粒细胞中的含量以及它们是否为嗜酸性粒细胞所特有，我们使用特异性放射免疫分析法测量了它们在人粒细胞裂解物中的浓度。我们还研究了不同的嗜酸性粒细胞裂解方法对这些蛋白回收率的影响。MBP在pH 2时回收率最高，其他颗粒蛋白在pH 5.6时回收率最高。这些蛋白与嗜酸性粒细胞共同沉降，其在每10⁶个嗜酸性粒细胞中的浓度（平均值±标准误，单位为ng/10⁶嗜酸性粒细胞及nM/10⁶嗜酸性粒细胞）分别为：MBP，8982±611（641.6）；EDN，3283±116（178.4）；ECP，5269±283（250.9）；EPO，12174±859（171.5）。一名正常人的嗜碱性粒细胞含有（单位为ng/10⁶细胞）：MBP，2374；EDN，214；ECP，77；EPO，17。高度纯化的中性粒细胞含有（单位为ng/10⁶细胞）：MBP，3±0.5；EDN，72±9；ECP，50±12。因此我们得出结论，这些蛋白主要在嗜酸性粒细胞中表达，但某些蛋白也存在于嗜碱性粒细胞和中性粒细胞中。
