Vollmer K L, Alberts J S, Carper H T, Mandell G L
Division of Infectious Diseases, University of Virginia Health Sciences Center, Charlottesville.
J Leukoc Biol. 1992 Dec;52(6):630-6. doi: 10.1002/jlb.52.6.630.
Tumor necrosis factor-alpha (TNF-alpha), a cytokine produced by mononuclear cells in response to endotoxin, inhibits neutrophil chemotaxis. We analyzed the effects of TNF-alpha on the orientation and movement of individual neutrophils in a chemoattractant gradient. Neutrophils, treated or untreated with TNF-alpha, were observed migrating in a gradient of the chemotactic peptide N-formyl-1-methionyl-1-leucyl-1-phenylalanine (fMLP) on a specially constructed chamber (Zigmond bridge). The movement of these cells was videotaped, digitized, and then tracked using a newly designed computer algorithm. The data obtained from this algorithm were then utilized to calculate distance traveled, speed and ability to polarize and migrate in a directed manner for each individual cell. TNF-alpha-treated cells behaved like cells not exposed to fMLP in that they failed to orient in a chemotactic gradient and moved in a manner similar to randomly migrating cells. This study provides unique observations of the effect of TNF-alpha on multiple parameters of PMN migration.
肿瘤坏死因子-α（TNF-α）是单核细胞对内毒素产生反应而分泌的一种细胞因子，它可抑制中性粒细胞趋化作用。我们分析了TNF-α对单个中性粒细胞在趋化因子梯度中定向和移动的影响。将经TNF-α处理或未处理的中性粒细胞置于特制的小室（齐格蒙德桥）中，使其在趋化肽N-甲酰-1-甲硫氨酰-1-亮氨酰-1-苯丙氨酸（fMLP）梯度中迁移，观察这些细胞的移动情况。将这些细胞的移动过程录像、数字化，然后使用新设计的计算机算法进行追踪。从该算法获得的数据随后用于计算每个细胞的移动距离、速度以及定向极化和迁移的能力。经TNF-α处理的细胞表现得就像未接触fMLP的细胞一样，它们无法在趋化梯度中定向，移动方式类似于随机迁移的细胞。本研究提供了关于TNF-α对PMN迁移多个参数影响的独特观察结果。
