Ghislain M, Spooner D M, Rodríguez F, Villamón F, Núñez J, Vásquez C, Waugh R, Bonierbale M
International Potato Center, P.O. Box 1558, Lima 12, Peru.
Theor Appl Genet. 2004 Mar;108(5):881-90. doi: 10.1007/s00122-003-1494-7. Epub 2003 Nov 27.
Characterization of nearly 1,000 cultivated potato accessions with simple sequence repeats (SSRs; also referred to as microsatellites) has allowed the identification of a reference set of SSR markers for accurate and efficient genotyping. In addition, 31 SSRs are reported here for a potato genetic map, including new map locations for 24 of them. A first criterion for this proposed reference set was ubiquity of the SSRs in the eight landrace cultivar groups of the potato, Solanum tuberosum. All SSRs tested in the present study displayed the same allele phenotypes and allele size range in the diverse germplasm set as in the advanced potato cultivar germplasm in which they were originally discovered. Thirteen of 13 SSR products from all cultivar groups are shown to cross-hybridize with the corresponding SSR product of the source cultivar to ascertain sequence homology. Other important SSR selection criteria are quality of amplification products, locus complexity, polymorphic index content, and well-dispersed location on a potato genetic map. Screening of 156 SSRs allowed the identification of a highly informative and user-friendly set comprising 18 SSR markers for use in characterization of potato genetic resources. In addition, we have identified true- and pseudo-multiplexing SSRs for even greater efficiency.
利用简单序列重复（SSR，也称为微卫星）对近1000份栽培马铃薯种质进行特征分析，已鉴定出一组用于准确高效基因分型的SSR标记参考集。此外，本文报道了用于马铃薯遗传图谱的31个SSR，其中24个有新的图谱定位。该提议的参考集的首要标准是SSR在马铃薯的8个地方品种组中的普遍性。本研究中测试的所有SSR在不同种质组中显示出与最初发现它们的高级马铃薯栽培种种质相同的等位基因表型和等位基因大小范围。来自所有品种组的13个SSR产物中有13个显示与源品种的相应SSR产物交叉杂交，以确定序列同源性。其他重要的SSR选择标准包括扩增产物质量、位点复杂性、多态性指数含量以及在马铃薯遗传图谱上分布良好的位置。对156个SSR进行筛选，鉴定出一组信息量丰富且用户友好的18个SSR标记，用于马铃薯遗传资源的特征分析。此外，我们还鉴定出了真多重和假多重SSR，以提高效率。
