流动注射化学发光法结合BrO₃⁻-奎宁体系测定氨基酸混合物及人尿中的L-半胱氨酸
Flow injection chemiluminescence determination of L-cysteine in amino acid mixture and human urine with the BrO3--quinine system.
作者信息
Li Baoxin, Zhang Zhujun, Liu Meilin, Xu Chunli
机构信息
Department of Chemistry, School of Chemistry and Materials Sciences, Shaanxi Normal University, 710062, Xi'an, P.R. China.
出版信息
Anal Bioanal Chem. 2003 Dec;377(7-8):1212-6. doi: 10.1007/s00216-003-2206-5. Epub 2003 Sep 16.
In this paper, a novel flow injection chemiluminescence (CL) determination of L-cysteine is proposed. The method is based on the CL reaction of L-cysteine and KBrO3 in acidic medium. The CL intensity was greatly enhanced in the presence of quinine. The CL intensity was linear with L-cysteine concentration in the range of 0.2-80 microg L(-1), and the detection limit was 0.1 microg L(-1) (3 sigma). A complete analysis, including sampling and injecting, could be performed in 1 min, giving a throughput of about 60 h(-1). The relative standard deviation was 1.6% for 0.8 microg L(-1 ) L-cysteine ( n=11). The proposed method was satisfactorily applied to the determination of cysteine in an amino acid mixture and human urine. The mechanism of the CL reaction is also discussed.
本文提出了一种流动注射化学发光法测定L-半胱氨酸的新方法。该方法基于L-半胱氨酸与KBrO₃在酸性介质中的化学发光反应。在奎宁存在下，化学发光强度大大增强。化学发光强度与L-半胱氨酸浓度在0.2 - 80 μg L⁻¹范围内呈线性关系，检测限为0.1 μg L⁻¹（3σ）。包括采样和进样在内的一次完整分析可在1分钟内完成，分析通量约为60 h⁻¹。对于0.8 μg L⁻¹的L-半胱氨酸，相对标准偏差为1.6%（n = 11）。该方法成功应用于氨基酸混合物和人尿中半胱氨酸的测定。还讨论了化学发光反应的机理。
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