Canapp Sherman O, Farese James P, Schultz Gregory S, Gowda Santosh, Ishak Anthony M, Swaim Steven F, Vangilder James, Lee-Ambrose Linda, Martin Frank G
Department of Small Animal Clinical Sciences, University of Florida College of Veterinary Medicine, and the School of Medicine Institute for Wound Research, Gainesville, FL 32610, USA.
Vet Surg. 2003 Nov-Dec;32(6):515-23. doi: 10.1111/j.1532-950x.2003.00515.x.
To evaluate the effects of topical glycyl-L-histidyl-L-lysine tripeptide-copper complex (TCC; Iamin 2% Gel; Procyte Corporation, Redmond, WA) on healing in ischemic open wounds.
Experimental study.
Twenty-four adult male Sprague-Dawley rats.
Rats were divided into 3 groups: topical TCC, topical TCC vehicle (hydroxypropyl-methylcellulose), and no treatment (control). Six-mm-diameter, full-thickness wounds were created within an ischemic bipedicle skin flap on the dorsum of each rat. Each day, for 13 days, wound margins were traced, and the TCC and TCC vehicle groups were treated topically. Tracings were scanned, and wound perimeter and area were calculated. On days 6, 10, and 13, selected wounds were biopsied and analyzed for tumor necrosis factor alpha (TNF-alpha) and matrix metalloproteinases (MMP) 2 and 9.
A significant decrease in wound area was seen in the TCC group, but not the vehicle group, when compared with the control group on days 3 to 5, 6 to 9, and 11 to 13 and when TCC was compared with TCC vehicle on days 3 and 9. On day 13, initial wound area had decreased by 64.5% in the TCC group, 45.6% in the vehicle group, and 28.2% in the control group. On days 6, 10, and 13, TCC-treated wounds contained significantly lower concentrations of TNF-alpha and MMP-2 and MMP-9 than control wounds.
Topical TCC resulted in accelerated wound healing in ischemic open wounds.
Topical TCC is an effective stimulant of healing of ischemic open wounds in rats and may have an application for the treatment of chronic wounds in other species. Clinical evaluation of topical TCC is warranted.
评估局部应用甘氨酰-L-组氨酰-L-赖氨酸三肽铜络合物（TCC；Iamin 2%凝胶；Procyte公司，华盛顿州雷德蒙德）对缺血开放性伤口愈合的影响。
实验研究。
24只成年雄性斯普拉格-道利大鼠。
将大鼠分为3组：局部应用TCC组、局部应用TCC赋形剂（羟丙基甲基纤维素）组和未治疗组（对照组）。在每只大鼠背部的缺血双蒂皮瓣上制作直径6毫米的全层伤口。连续13天，每天描绘伤口边缘，并对TCC组和TCC赋形剂组进行局部治疗。扫描描绘图，计算伤口周长和面积。在第6、10和13天，对选定的伤口进行活检，分析肿瘤坏死因子α（TNF-α）以及基质金属蛋白酶（MMP）2和9。
与对照组相比，在第3至5天、第6至9天以及第11至13天，TCC组伤口面积显著减小，而赋形剂组未减小；在第3天和第9天，TCC组与TCC赋形剂组相比伤口面积也显著减小。在第13天，TCC组初始伤口面积减少了64.5%，赋形剂组减少了45.6%，对照组减少了28.2%。在第6、10和13天，TCC治疗的伤口中TNF-α、MMP-2和MMP-9的浓度显著低于对照伤口。
局部应用TCC可加速缺血开放性伤口的愈合。
局部应用TCC是大鼠缺血开放性伤口愈合的有效刺激剂，可能适用于其他物种慢性伤口的治疗。有必要对局部应用TCC进行临床评估。
