一种从软骨硫酸角质素连接区分离还原糖片段的简单快速方法。
A simple and rapid method for isolation of reduced carbohydrate fragments from the linkage region of cartilage keratan sulphate.
作者信息
Kieras F J
出版信息
Biochem J. 1977 Dec 1;167(3):851-3. doi: 10.1042/bj1670851.
Abstract
The method described is a simple and rapid procedure for isolation in high yield of carbohydrate fragments containing terminal galactosaminitol derived from the polysaccharide-protein linkage region of cartilage keratan sulphate. It is based on the observation that reducing sugars bind tightly to Dowex-1 resin (hydroxide form), whereas reduced analogues (sugar alcohols) do not [H. Yamaguchi, S. Inamura & K. Makino (1976) J. Biochem. (Tokyo) 79, 299-303].
摘要
所描述的方法是一种简单快速的程序，用于从软骨硫酸角质素的多糖-蛋白质连接区域高产率地分离出含有末端半乳糖胺醇的碳水化合物片段。该方法基于这样的观察结果：还原糖紧密结合到Dowex-1树脂（氢氧化物形式）上，而还原类似物（糖醇）则不会[H. 山口、S. 稻村和K. 牧野（1976年）《生物化学杂志》（东京）79, 299 - 303]。
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A simple and rapid method for isolation of reduced carbohydrate fragments from the linkage region of cartilage keratan sulphate.一种从软骨硫酸角质素连接区分离还原糖片段的简单快速方法。
Biochem J. 1977 Dec 1;167(3):851-3. doi: 10.1042/bj1670851.
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Physical properties and polydispersity of proteoglycan from bovine nasal cartilage.来自牛鼻软骨的蛋白聚糖的物理性质和多分散性
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The structure of keratan sulphates from various sources.来自不同来源的硫酸角质素的结构。
Biochem J. 1975 Dec;151(3):543-53. doi: 10.1042/bj1510543.
6
Alkaline borohydride treatment of cartilage keratan sulfate: a comparison of two reaction conditions.软骨硫酸角质素的碱性硼氢化物处理：两种反应条件的比较
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Studies on a microchemical method for the determination of the degree of polymerization of neutral oligo- and polysaccharides. I. Quantitative separation of trace amounts of alditols from mixtures with a large excess of monosaccharides.中性寡糖和多糖聚合度测定的微量化学方法研究。I. 从大量单糖混合物中定量分离痕量糖醇。
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