软骨硫酸角质素的结构与组成。
The structure and composition of cartilage keratan sulphate.
作者信息
Hopwood J J, Robinson H C
出版信息
Biochem J. 1974 Aug;141(2):517-26. doi: 10.1042/bj1410517.
Abstract
Keratan sulphate was isolated from bovine intervertebral disc and bovine nasal septum after hydrolysis with proteinases and treatment with dilute alkali. Each preparation was found to contain, per keratan sulphate chain: (a) 1 residue of mannose; (b) 3 residues of N-acetylneuraminic acid (2 residues after alkali treatment); (c) 1 residue of N-acetylgalactosamine (lost after alkali treatment); (d) 1 residue or less of fucose. N-Acetyl-neuraminic acid residues were at non-reducing termini and were bonded to keratan sulphate through galactose residues. Evidence is presented for two different types of linkage between skeletal keratan sulphate and protein. Consideration of molecular parameters and compositions leads to a proposed structure for keratan sulphate-protein as found in skeletal proteoglycans.
摘要
硫酸角质素是在经蛋白酶水解并用稀碱处理后，从牛椎间盘和牛鼻中隔中分离得到的。发现每种制剂中，每条硫酸角质素链含有：(a) 1个甘露糖残基；(b) 3个N-乙酰神经氨酸残基（碱处理后为2个残基）；(c) 1个N-乙酰半乳糖胺残基（碱处理后丢失）；(d) 1个或更少的岩藻糖残基。N-乙酰神经氨酸残基位于非还原末端，并通过半乳糖残基与硫酸角质素相连。文中提供了关于骨架硫酸角质素与蛋白质之间两种不同连接类型的证据。对分子参数和组成的考虑得出了在骨架蛋白聚糖中发现的硫酸角质素-蛋白质的推测结构。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
THE ROLE OF GALACTOSE AND XYLOSE IN THE LINKAGE OF HEPARIN TO PROTEIN.半乳糖和木糖在肝素与蛋白质连接中的作用。
J Biol Chem. 1965 Jul;240:2821-6.
3
VARIATIONS IN KERATOSULFATES.硫酸角质素的变异
J Biol Chem. 1965 Mar;240:1005-10.
4
THE EXTRACELLULAR GLYCOSIDASES OF DIPLOCOCCUS PNEUMONIAE. I. PURIFICATION AND PROPERTIES OF A NEURAMINIDASE AND A BETA-GALACTOSIDASE. ACTION ON THE ALPHA-1-ACID GLYCOPROTEIN OF HUMAN PLASMA.肺炎双球菌的细胞外糖苷酶。I. 一种神经氨酸酶和一种β-半乳糖苷酶的纯化及特性。对人血浆α-1-酸性糖蛋白的作用
Biochemistry. 1964 Oct;3:1535-43. doi: 10.1021/bi00898a025.
5
Quantitative estimation of sialic acids. II. A colorimetric resorcinol-hydrochloric acid method.唾液酸的定量测定。II. 比色间苯二酚 - 盐酸法。
Biochim Biophys Acta. 1957 Jun;24(3):604-11. doi: 10.1016/0006-3002(57)90254-8.
6
Studies on keratosulfates. Methylation and partial acid hydrolysis of bovine corneal keratosulfate.硫酸角质素的研究。牛眼角膜硫酸角质素的甲基化和部分酸水解。
J Biol Chem. 1967 Oct 10;242(19):4352-9.
7
The proteinpolysaccharides of bovine nucleus pulposus.牛椎间盘髓核的蛋白多糖
J Biol Chem. 1967 Oct 25;242(20):4691-701.
8
Action of degradative enzymes on the light fraction of bovine septa protein polysaccharide.降解酶对牛鼻中隔蛋白多糖轻组分的作用
Biochem J. 1967 Apr;103(1):103-9. doi: 10.1042/bj1030103.
9
Studies on porcine costal cartillage protein-polysaccharide complex. I. Enzymatic degradation.
Biochim Biophys Acta. 1966 May 26;121(1):120-7. doi: 10.1016/0304-4165(66)90354-0.
10
Determination of galactosamine and N-acetylgalactosamine in the presence of other hexosamines with galactose oxidase.在存在其他己糖胺的情况下，用半乳糖氧化酶测定半乳糖胺和N-乙酰半乳糖胺。
Anal Biochem. 1965 Sep;12(3):509-15. doi: 10.1016/0003-2697(65)90217-4.
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