Hopwood J J, Robinson H C
Biochem J. 1974 Aug;141(2):517-26. doi: 10.1042/bj1410517.
Keratan sulphate was isolated from bovine intervertebral disc and bovine nasal septum after hydrolysis with proteinases and treatment with dilute alkali. Each preparation was found to contain, per keratan sulphate chain: (a) 1 residue of mannose; (b) 3 residues of N-acetylneuraminic acid (2 residues after alkali treatment); (c) 1 residue of N-acetylgalactosamine (lost after alkali treatment); (d) 1 residue or less of fucose. N-Acetyl-neuraminic acid residues were at non-reducing termini and were bonded to keratan sulphate through galactose residues. Evidence is presented for two different types of linkage between skeletal keratan sulphate and protein. Consideration of molecular parameters and compositions leads to a proposed structure for keratan sulphate-protein as found in skeletal proteoglycans.
硫酸角质素是在经蛋白酶水解并用稀碱处理后，从牛椎间盘和牛鼻中隔中分离得到的。发现每种制剂中，每条硫酸角质素链含有：(a) 1个甘露糖残基；(b) 3个N-乙酰神经氨酸残基（碱处理后为2个残基）；(c) 1个N-乙酰半乳糖胺残基（碱处理后丢失）；(d) 1个或更少的岩藻糖残基。N-乙酰神经氨酸残基位于非还原末端，并通过半乳糖残基与硫酸角质素相连。文中提供了关于骨架硫酸角质素与蛋白质之间两种不同连接类型的证据。对分子参数和组成的考虑得出了在骨架蛋白聚糖中发现的硫酸角质素-蛋白质的推测结构。
