Verettas Dionisios, Kazakos Costas, Tilkeridis Costas, Dermon Antonios, Petrou Harris, Galanis Vassilios
Orthopaedic Department, Democritus University of Thrace, Medical School, Alexandroupolis Regional Hospital, Greece.
Acta Orthop Belg. 2003 Oct;69(5):396-9.
Extrapulmonary tuberculosis accounts for approximately 10% of tuberculous infections; the musculoskeletal system is involved in a small number of these (10%). Skeletal tuberculosis is an indolent disease, and diagnosis may be delayed. Conventional methods are time consuming and have a low sensitivity rate. In recent years PCR-based protocols raised hopes as a reliable and fast diagnostic tool for extrapulmonary tuberculosis. The authors report the detection of Mycobacterium tuberculosis complex DNA in specimens from six patients using a nested PCR protocol specific for IS6110 insertion element of Mycobacterium tuberculosis complex. Three men and three women are reported with ages ranging from 42 to 68 years. The sites of infection were the knee and shoulder in one case each, the hip in two cases, and the thoracic spine in two cases. Diagnosis was established within three days, and treatment was initiated promptly. PCR is a technically easy approach that can be used as a first step diagnostic tool for early recognition and treatment of bone and joint tuberculosis.
肺外结核约占结核感染的10%；其中少数病例（10%）累及肌肉骨骼系统。骨结核是一种进展缓慢的疾病，诊断可能会延迟。传统方法耗时且灵敏度低。近年来，基于聚合酶链反应（PCR）的检测方案有望成为一种可靠且快速的肺外结核诊断工具。作者报告了使用针对结核分枝杆菌复合群IS6110插入元件的巢式PCR方案，在6例患者的标本中检测到结核分枝杆菌复合群DNA。报告的6例患者中，3男3女，年龄在42至68岁之间。感染部位分别为1例膝关节、1例肩关节、2例髋关节和2例胸椎。在三天内确诊，并立即开始治疗。PCR是一种技术上简单的方法，可作为早期识别和治疗骨与关节结核的第一步诊断工具。
