Das B K, Gurubacharya Rajesh Lal, Mohapatra T M, Mishra O P
Department of Pediatrics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India.
Indian J Pediatr. 2003 Oct;70(10):799-801. doi: 10.1007/BF02723800.
To evaluate the role of bacterial antigen detection test in cerebrospinal fluid (CSF) for a rapid etiological diagnosis of bacterial meningitis.
The study included 36 cases of bacterial meningitis and 14 controls. Latex particle agglutination test (LPA test) for detection of bacterial antigen was done in the CSF using slidex meningitis kit (Biomeriux, France).
Using LPA test, an etiological diagnosis could be made in 83% cases of bacterial meningitis. In contrast, CSF Gram stain and culture showed 36% and 6% positivity, respectively. The sensitivity and specificity of LPA test were 83% and 100%, respectively. The common etiological organisms were S. pneumoniae, H. influenzae type b and N. meningitidis A. S. pneumoniae was encountered in all age groups while H. influenzae type b was found only below one year of age.
LPA test is a rapid and superior diagnostic tool as compared to CSF Gram stain and culture. The study recommends LPA test as an adjunct laboratory test for rapid etiological diagnosis of bacterial meningitis for prompt institution of proper antibiotics.
评估脑脊液（CSF）细菌抗原检测试验在细菌性脑膜炎快速病因诊断中的作用。
该研究纳入36例细菌性脑膜炎患者和14例对照。使用法国生物梅里埃公司的思乐智脑膜炎试剂盒，通过乳胶颗粒凝集试验（LPA试验）检测脑脊液中的细菌抗原。
采用LPA试验，83%的细菌性脑膜炎病例可做出病因诊断。相比之下，脑脊液革兰氏染色和培养的阳性率分别为36%和6%。LPA试验的敏感性和特异性分别为83%和100%。常见的病原体为肺炎链球菌、b型流感嗜血杆菌和A群脑膜炎奈瑟菌。各年龄组均有肺炎链球菌感染，而b型流感嗜血杆菌仅见于1岁以下儿童。
与脑脊液革兰氏染色和培养相比，LPA试验是一种快速且更优的诊断工具。该研究推荐将LPA试验作为细菌性脑膜炎快速病因诊断的辅助实验室检查，以便及时应用恰当的抗生素。
