Fan Zheng, Tan Xin-yu, Yin Bin, Zou Ke, Wang Ting, Shen Yan, Ni An-ping, Qin Chuan, Yuan Jian-gang, Qiang Bo-qin, Peng Xiao-zhong
National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 100005, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Oct;25(5):499-503.
To get the cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain.
Using the SARS-CoV PUMC2 strain genomic RNA as the template, the cDNA fragments were amplified by RT-PCR, the PCR products were further purified and ligated into the pGEM-T vector, and all the clones obtained were sequenced.
The cDNA clones which cover the whole genome of SARS-CoV PUMC2 strain were obtained.
These cDNAs can be provided for the function study of SARS-CoV proteins and the construction of full-length infectious cDNA clone of SARS-CoV.
获取覆盖严重急性呼吸综合征冠状病毒（SARS-CoV）PUMC2株全基因组的cDNA克隆。
以SARS-CoV PUMC2株基因组RNA为模板，通过逆转录聚合酶链反应（RT-PCR）扩增cDNA片段，进一步纯化PCR产物并连接到pGEM-T载体中，对获得的所有克隆进行测序。
获得了覆盖SARS-CoV PUMC2株全基因组的cDNA克隆。
这些cDNA可用于SARS-CoV蛋白的功能研究及SARS-CoV全长感染性cDNA克隆的构建。
