Lu Zhao-hui, Chen Jie, Luo Yu-feng, Cao Jin-ling, Wan Jian-wei, Wang De-tian, Zhang Hong-tu, Xie Yong-qiang
Department of Pathology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Oct;25(5):508-11.
To investigate the roles of different cells in the pulmonary lesions in the severe acute respiratory syndrome (SARS) patients.
The monoclonal antibodies of CD8, CD20, CD34, LCA, CD56, CD68, and AE1/AE3 are used to demonstrate the different cells in the lung specimens of SARS patients in order to study the patterns of cell responses in this new disease. Meanwhile the HE stained slides were also carefully studied to compare with the results of immunohistochemical staining.
The number of capillaries increased and the capillaries clearly outlined the contour of alveolar wall from beginning to early stage of organization, the number of lymphocytes decreased sharply while the number of macrophage remarkably increased, together with proliferation of type II pneumocytes. The numbers of blood vessels decreased in the fibrotic and consolidated lung tissue, and the vessel cavities enlarged, losing the normal contour of alveolar septa.
The lesions in the lung from SARS patients are consisted of the tissue reaction to the inflammatory injury, including extensive exudation, capillary proliferation, fibrosis, and obvious infiltration of macrophages which may play a key role in the pathogenesis of pulmonary lesions of SARS.
探讨不同细胞在严重急性呼吸综合征（SARS）患者肺部病变中的作用。
采用CD8、CD20、CD34、LCA、CD56、CD68及AE1/AE3单克隆抗体，对SARS患者肺组织标本中的不同细胞进行显示，以研究这种新疾病中的细胞反应模式。同时，仔细研究苏木精-伊红（HE）染色切片，与免疫组织化学染色结果进行比较。
从病变开始至组织化早期，毛细血管数量增多，清晰勾勒出肺泡壁轮廓，淋巴细胞数量急剧减少，巨噬细胞数量显著增加，同时Ⅱ型肺泡上皮细胞增生。在纤维化和实变肺组织中血管数量减少，血管腔扩大，肺泡间隔失去正常轮廓。
SARS患者肺部病变包括对炎症损伤的组织反应，如广泛渗出、毛细血管增生、纤维化，以及巨噬细胞明显浸润，巨噬细胞可能在SARS肺部病变发病机制中起关键作用。
