Iida S, Saito M, Okazaki T, Seto M, Yamamoto K, Akao Y, Ogura M, Suzuki H, Ariyoshi Y, Koike K
Department of Hematology and Chemotherapy, Aichi Cancer Center, Japan.
Leuk Res. 1992 Dec;16(12):1155-63. doi: 10.1016/0145-2126(92)90113-l.
11q23 translocation is the most popular chromosomal abnormality in infant leukemia. In adults, it is often encountered in non-Hodgkin's lymphoma (NHL). In this study, we analyzed the phenotypic and genotypic characteristics of 9 acute leukemic cell lines with 11q23 translocations and one with deletion of the 11q23 locus, nine of which were established by researchers in this group, together with 4 NHL cell lines with 11q23 translocations. All lines were considered to belong to the B-cell lineage at different stages. All 10 leukemic lines showed clonal rearrangement of the immunoglobulin heavy chain (IgH) gene: two corresponded to the B-precursor stage (CD19+, cytoplasmic mu-), while the other 8 corresponded to the pre-B stage (cytoplasmic mu+). All 4 NHL lines showed rearrangements of both the IgH and Ig kappa genes with three expressing surface Ig; specifically, mature B-cell phenotype. As for myelocytic-monocytic markers, at least one out of 4 antigens examined were positive in 8 of the 10 leukemic cell lines, while only one of the 4 NHL lines was reactive. There were essentially no clear phenotypic or genotypic differences between t(4;11) and t(11;19) cell lines, supporting the view that both diseases have similar clinicopathological characteristics. These cell lines are also valuable for cloning genes at the chromosomal breakpoints.
11q23易位是婴儿白血病中最常见的染色体异常。在成人中，它常出现在非霍奇金淋巴瘤（NHL）中。在本研究中，我们分析了9个具有11q23易位的急性白血病细胞系和1个具有11q23位点缺失的细胞系的表型和基因型特征，其中9个是由本研究团队的研究人员建立的，同时分析了4个具有11q23易位的NHL细胞系。所有细胞系均被认为属于不同阶段的B细胞谱系。所有10个白血病细胞系均显示免疫球蛋白重链（IgH）基因的克隆性重排：2个对应B前体细胞阶段（CD19 +，胞质μ-），而另外8个对应前B细胞阶段（胞质μ+）。所有4个NHL细胞系均显示IgH和Igκ基因的重排，其中3个表达表面Ig；具体而言，为成熟B细胞表型。至于髓细胞-单核细胞标志物，在10个白血病细胞系中的8个中，所检测的4种抗原中至少有一种呈阳性，而4个NHL细胞系中只有一个有反应。t(4;11)和t(11;19)细胞系之间基本上没有明显的表型或基因型差异，这支持了两种疾病具有相似临床病理特征的观点。这些细胞系对于在染色体断点处克隆基因也很有价值。
