丘吉尔是一种锌指转录激活因子，可调节原肠胚形成和神经胚形成之间的转变。

Churchill, a zinc finger transcriptional activator, regulates the transition between gastrulation and neurulation.

作者信息

Sheng Guojun, dos Reis Mario, Stern Claudio D

机构信息

Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, United Kingdom.

出版信息

Cell. 2003 Nov 26;115(5):603-13. doi: 10.1016/s0092-8674(03)00927-9.

DOI:10.1016/s0092-8674(03)00927-9

PMID:14651851

Abstract

Gastrulation generates mesoderm and endoderm from embryonic epiblast; soon after, the neural plate is established within the epiblast-both events require FGF signaling. We describe a zinc finger transcriptional activator, Churchill (ChCh), which acts as a switch between different roles of FGF. FGF induces ChCh slowly; this activates Smad-interacting-protein-1 (Sip1), which blocks further induction of the mesoderm markers brachyury and Tbx6L by FGF. ChCh is first expressed as cells stop migrating through the primitive streak, and we show that it regulates cell ingression. We propose a simple mechanism by which FGF sensitizes cells to BMP signals. These results reveal that neural induction requires cessation of mesoderm formation at the midline in addition to the decision between epidermis and neural plate.

摘要

原肠胚形成过程中，胚胎上胚层产生中胚层和内胚层；此后不久，上胚层内形成神经板——这两个过程都需要FGF信号传导。我们描述了一种锌指转录激活因子丘吉尔（ChCh），它在FGF的不同作用之间起开关作用。FGF缓慢诱导ChCh；这会激活Smad相互作用蛋白1（Sip1），从而阻断FGF对中胚层标志物短尾相关蛋白（brachyury）和Tbx6L的进一步诱导。ChCh最初在细胞停止通过原条迁移时表达，我们发现它调节细胞内陷。我们提出了一种简单的机制，通过该机制FGF使细胞对BMP信号敏感。这些结果表明，神经诱导除了需要决定表皮和神经板之间的分化外，还需要在中线处停止中胚层的形成。